J4 ›› 2009, Vol. 47 ›› Issue (05): 1077-1080.
Previous Articles Next Articles
WANG Ji, GAO Ruijuan, YUAN Dawei, WANG Li
Received:
Online:
Published:
Contact:
Abstract:
p53 cDNA was obtained from human peripheral blood lymphocytes by RTPCR, and then the p53 fragments were inserted into pQE40 vector to construct the recombinant plasmid pQE40p53. Furthermore, pQE40p53 plasmids were transformed into E.coli M15, and then induced by 1 mmol/L IPTG to express p53 protein in E.coli M15 expression system. The recombinant p53 protein fused with 6His-tag was purified by Ni-NAT resin affinity chromatography, and then urea was removed with gradual dialysis. Western Blot confirmed that purified 6His-p53 recombinant protein was obtained.
Key words: p53 gene, gene cloning, prokaryotic expression
CLC Number:
WANG Ji, GAO Rui-Juan, YUAN Da-Wei, WANG Li. Cloning and Prokaryotic Expression of p53 Gene[J].J4, 2009, 47(05): 1077-1080.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/lxb/EN/
http://xuebao.jlu.edu.cn/lxb/EN/Y2009/V47/I05/1077
Cited