J4 ›› 2009, Vol. 47 ›› Issue (05): 1081-1085.

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Expression, Purification and Refolding of Recombinant MatrixMetalloproteinase MT5MMP

SHI Xiujuan, JIANG Kun, FANG Xuexun   

  1. Key Laboratory for Molecular Enzymology and Enzyme Engineering of the Ministry of Education,Jilin University, Changchun 130021, China
  • Received:2009-01-23 Online:2009-09-26 Published:2009-11-03
  • Contact: FANG Xuexun E-mail:fangxx@jlu.edu.cn.

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Abstract:

The authors completed the induction expression of recombinant MT5-MMP in E.coli BL21(DE3) and harvested the protein as inclusion bodies. The molecular weight of the protein is about 21 000. In addition, we purified and refolded the recombinant protein using the chromatography method to obtain the catalytically active MT5-MMP.

Key words: matrix metalloproteinase (MMP), inclusion bodies, renaturation

CLC Number: 

  • Q55
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