Abstract:

To establish an HPLC method for the determination of panasenoside ［kaempferol-3-O-β-D-glucopyranosyl-（1→2）-β-D-galactopyranoside］ in the leaves of Panax ginseng C.A.Meyer, the chromatographic separation was performed on a Zorbax Extend C18 column (250 mm×4.6 mm, 5 μm), with V(methanol) ∶V(acetonitrile) ∶ V(0.1% acetic acid/water)=25 ∶5∶70 as mobile phase at a rate of 1.2 mL/min and 25 ℃. The detection wavelength was set at 268 nm. The results show that the linearity of panasenoside was in a range of 0.5～20 μg(r=0.999 9, n=6), and the average recovery and RSD were 98.5% and 064% respectively (n=6).

Key words: Panax ginseng C.A.Meyer, panasenoside, high performance liquid chromatography (HPLC), determination