关键词: 甲状腺乳头状肿瘤；免疫组织化学；酶联免疫法；实时荧光定量法；白细胞介素1&alpha, ；白细胞介素1&beta, ；环氧合酶-2

Abstract:

Objective To investigate the expressions of inflammatory factors in papillary thyroid carcinoma tissue and their relationships with the clinical pat
hological characteristics,and to provide an experimental basis for guiding clinical treatment and prognosis.Methods 74 patients with papillary thyroid carcinoma dignosed by puncture were selected as experimental group,and  26 cases of healthy people were used as normal control group.The fasting venous blood samples  from the subjects in two groups were collected,and the postoperative specimens from cancer tissue,adjacent normal tissue and the fasting venous blood 7 d after operation of the patients in experimental group were collected again.The interleukin 1α (IL-1α),interleukin 1β (IL-1β) and cyclooxygenase -2 (COX-2) protein expression levels in serum,cancer adjacent normal tissue and cancer tissue were detected by ELISA;the gene and protein expression levels of IL-1α,IL-1β and COX-2 were examined by real-time fluorescent quantitative method and immunohistochemistry;th
e relationships between their expressions and clinical stages,pathological types, lymph node metastasis of thyroid papillary carcinoma were analyzed.
Results The expression levels of serum IL-1α,IL-1β and COX-2 protein of the patients in experimental group  were significantly higher than those in normal
 control group(P<0.01);the gene and protein expression levels of IL-1α,IL-1β and COX-2 in thyroid papillary carcinoma tissue were significantly
 higher than those in adjacent tissue (P<0.01).There were  positive correlations between the expressions of IL-1α and IL-1β,IL-1α and COX-2,IL-1β and
 COX-2 in thyroid papillary carcinoma tissue (r=0.64,P=0.035;r=0.71,P=0.042;r=0.69,P=0.038).Conclusion The expression levels of I
L-1α,IL-1β and COX-2 in thyroid papillary carcinoma tissue are increased,suggesting that inflammation is involved in metastasis and invasion  of thyroid papillary carcinoma,its application is expected to become a new means of cancer treatment strategies.

Key words: thyroid papillary neoplasms, immunohistochemistry, enzyme linked immunosorbent assay, real time fluorescent quantitative method, interleukin 1&alpha, interleukin 1&beta, cyclooxygenase-2