Wnt/β-catenin通路关键分子在NK/T细胞淋巴瘤组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20150205

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 230-234.doi: 10.13481/j.1671-587x.20150205

Wnt/β-catenin通路关键分子在NK/T细胞淋巴瘤组织中的表达及其临床意义

秦贝贝¹, 李亚青¹, 李小丽¹, 路礼莎², 张旭东², 张明智¹

1. 郑州大学第一附属医院淋巴瘤诊疗中心, 河南郑州 450052;
2. 河南省高等学校临床医学重点学科开放实验室, 河南郑州 450052

收稿日期:2014-07-24 出版日期:2015-03-28 发布日期:2015-04-04
通讯作者: 张明智, 主任医师, 博士研究生导师(Tel:0371-66295563, E-mail:mingzhi_zhang1@163.com) E-mail:mingzhi_zhang1@163.com
作者简介:秦贝贝(1989-), 女, 河南省驻马店市人, 在读医学硕士, 主要从事淋巴瘤分子机制方面的研究。
基金资助:
国家自然科学基金资助课题(81172118)

Expressions of key molecules of Wnt/β-catenin signal pathway in NK/T-cell lymphoma tissue and their significances

QIN Beibei¹, LI Yaqing¹, LI Xiaoli¹, LU Lisha², ZHANG Xudong², ZHANG Mingzhi¹

1. Lymphoma Diagnosis and Treatment Center, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
2. Institute of Clinical Medicine Department, Henan Province, Zhengzhou 450052, China

Received:2014-07-24 Online:2015-03-28 Published:2015-04-04

摘要/Abstract

摘要：

目的:研究Wnt/β-catenin通路的关键分子β-catenin、c-myc和cyclinD₁在NK/T细胞淋巴瘤(NKTCL)组织中的表达,阐明其与NKTCL患者临床特征的关系。方法:采用Real-time PCR法检测人NKTCL细胞株(SNK-6和YTS)及人正常NK细胞中Wnt/β-catenin信号通路相关基因β-catenin、c-myc和cyclinD₁ mRNA的表达水平;采用免疫组织化学SP法检测NKTCL组织和反应性增生的淋巴组织中β-catenin、c-myc蛋白的表达阳性率,并分析蛋白表达与患者临床特征的关系;分析NKTCL患者中β-catenin和c-myc蛋白表达的相关性;Kaplan-Meier法分析β-catenin表达与NKTCL患者中位生存期的关系。结果:细胞株SNK-6和YTS中β-catenin、c-myc和cyclinD₁ mRNA的表达阳性率明显高于正常NK细胞(P<0.05);NKTCL组织中β-catenin和c-myc蛋白表达阳性率(24%和56%)明显高于淋巴结反应性增生组织(0%和25%)(P<0.05);β-catenin表达与Ann Arbor分期有关联(P<0.05),c-myc表达与Ann Arbor分期和Ki-67水平有关联(P<0.05),NKTCL患者中β-catenin和c-myc表达呈正相关关系(r=0.770,P<0.05);β-catenin阳性表达患者中位生存期低于β-catenin阴性表达患者(P<0.05)。结论:Wnt/β-catenin信号通路中关键分子β-catenin、c-myc、cyclinD₁在NKTCL组织中高表达,并且其表达水平与NKTCL患者临床分期有关联。

关键词: NK/T细胞淋巴瘤, Wnt/β-catenin信号通路, β-catenin, c-myc, cyclinD₁

Abstract:

Objective To investigate the expressions of the key molecules β-catenin,c-myc,and cyclinD₁ of the Wnt/β-catenin signal pathway in the NK/T-cell lymphoma (NKTCL) tissue, and to clarify their relationships with the clinicopathological features of the NKTCL patients.Methods Real-time PCR method was used to detect the expression levels of β-catenin,c-myc,and cyclinD₁ mRNA in human NKTCL cell lines (SNK-6 and YTS) and normal NK cells;the positive expression rates of β-catenin and c-myc proteins in the NKTCL tissue and reactive hyperplasia of lymph node tissue were detected by immunohistochemistry SP method;the correlations among the expressions of β-catenin and c-myc proteins and the clinicopathological features of the NKTCL patients were analyzed;correlation analysis was used to analyze the relationship between the expressions of β-catenin and c-myc proteins of the NKTCL patients;Kaplan-Meier method was used to analyze the relationship between the expression of β-catenin and overall survival rate of the NKTCL patients.Results The positive expression rates of β-catenin,c-myc,and cyclinD₁ mRNA in the SNK-6 cell line and YTS cell line were significantly higher than those in the normal NK cells(P<0.05);the expression β-catenin and c-myc proteins in NKTCL tissue(24% and 56%) were higher than those in reactive hyperplasia of lymph node tissue (0 and 25%)(P<0.05);the expression of β-catenin was correlated with Ann Arbor stage(P<0.05),and the expression of c-myc was correlated with Ann Arbor stage and Ki-67(P<0.05);there were positive correlations between the expression of β-catenin and the expression of c-myc in the NKTCL patients(r=0.770,P<0.05);the patients with β-catenin positive expression had a shorter overall survival time than the patients with negative expression (P<0.05).Conclusion The key molecules β-catenin,c-myc,and cyclinD₁ of the Wnt/β-catenin signal pathway are highly expressed in the human NKTCL tissue,and they show positive correlations with Ann Arbor stage of the NKTCL patients.

Key words: NK/T cell lymphma, Wnt/β-catenin signal pathway, β-catenin, c-myc, cyclinD₁

中图分类号:

R733.4

秦贝贝, 李亚青, 李小丽, 路礼莎, 张旭东, 张明智. Wnt/β-catenin通路关键分子在NK/T细胞淋巴瘤组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2015, 41(02): 230-234.

QIN Beibei, LI Yaqing, LI Xiaoli, LU Lisha, ZHANG Xudong, ZHANG Mingzhi. Expressions of key molecules of Wnt/β-catenin signal pathway in NK/T-cell lymphoma tissue and their significances[J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 230-234.

参考文献

[1] Suzuki R.Extranodal NK/T-cell lymphoma[J].Nihon Rinsho,2014,72(3):524-530.

[2] MacDonald BT,Tamai K,He X.Wnt/β-catenin signaling:components,mechanisms,and diseases[J].Dev Cell,2009,17(1):9-26.

[3] Ge X,Lv X,Feng L.High expression and nuclear localization of β-catenin in diffuse large B-cell lymphoma[J].SMol Med Rep,2012,5(6):1433-1437.

[4] Gelebart P,Anand M,Armanious H,et al.Constitutive activation of the Wnt canonical pathway in mantle cell lymphoma[J].Blood,2008,112(13):5171-5179.

[5] Kaveri D,Kastner P,Dembélé D,et al.β-Catenin activation synergizes with Pten loss and Myc overexpression in Notch-independent T-ALL[J].Blood,2013,122(5):694-704.

[6] Sharma A,Sen JM.Molecular basis for the tissue specificity of β-catenin oncogenesis[J].Oncogene,2013,32(15):1901-1909.

[7] Zhang XY,Wang X.Roles of signal transduction pathway in non-Hodgkin's lymphoma[J].Zhongguo Shi Yan Xue Za Zhi,2011,19(2):532-536.

[8] 麦玉洁,邱录贵,李增军,等.β-catenin在白血病细胞系中表达的研究[J].中国实验血液学杂志,2007,15(4):919-922.

[9] Bellei B,Pacchiarotti A,Perez M,et al.Frequent beta-catenin overexpression without exon 3 mutation in cutaneous lymphomas[J].Mod Pathol,2004,17(10):1275-1281.

[10] Huang X,Sun Q,Fu H,et al.Both c-Myc and Ki-67 expression are predictive markers in patients with Extranodal NK/T-cell lymphoma,nasal type:A retrospective study in China[J].Pathol Res Pract,2014,210(6):351-356.

[11] Ng SB,Selvarajan V,Huang G,et al.Activated oncogenic pathways and therapeutic targets in extranodal nasal-type NK/T cell lymphoma revealed by gene expression profiling[J].J Pathol,2011,223(4):496-510.

[12] Shao N,Zou J, Li J,et al.Hyper-activation of WNT/β-catenin signaling pathway mediates anti-tumor effects of histone deacetylase inhibitors in acute T lymphoblastic leukemia[J].Leuk Lymphoma,2012,53(9):1769-1778.

[13] Schmidt M,Sievers E,Endo T,et al.Targeting Wnt pathway in lymphoma and myeloma cells[J].Br J Haematol,2009,144(5):796-798.

[14] Kawahara T,Kawaguchi-Ihara N,Okuhashi Y,et al.Cyclopamine and quercetin suppress the growth of leukemia and lymphoma cells[J].Anticancer Res,2009,29(11):4629-4632.

[15] Li S,Feng X,Li T, et al.Extranodal NK/T-cell lymphoma,nasal type:a report of 73 cases at MD Anderson Cancer Center[J].Am J Surg Pathol,2013,37(1):14-23.

Wnt/β-catenin通路关键分子在NK/T细胞淋巴瘤组织中的表达及其临床意义

Expressions of key molecules of Wnt/β-catenin signal pathway in NK/T-cell lymphoma tissue and their significances

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