吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 230-234.doi: 10.13481/j.1671-587x.20150205

• 基础研究 • 上一篇    下一篇

Wnt/β-catenin通路关键分子在NK/T细胞淋巴瘤组织中的表达及其临床意义

秦贝贝1, 李亚青1, 李小丽1, 路礼莎2, 张旭东2, 张明智1   

  1. 1. 郑州大学第一附属医院淋巴瘤诊疗中心, 河南 郑州 450052;
    2. 河南省高等学校临床医学重点学科开放实验室, 河南 郑州 450052
  • 收稿日期:2014-07-24 出版日期:2015-03-28 发布日期:2015-04-04
  • 通讯作者: 张明智, 主任医师, 博士研究生导师(Tel:0371-66295563, E-mail:mingzhi_zhang1@163.com) E-mail:mingzhi_zhang1@163.com
  • 作者简介:秦贝贝(1989-), 女, 河南省驻马店市人, 在读医学硕士, 主要从事淋巴瘤分子机制方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81172118)

Expressions of key molecules of Wnt/β-catenin signal pathway in NK/T-cell lymphoma tissue and their significances

QIN Beibei1, LI Yaqing1, LI Xiaoli1, LU Lisha2, ZHANG Xudong2, ZHANG Mingzhi1   

  1. 1. Lymphoma Diagnosis and Treatment Center, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
    2. Institute of Clinical Medicine Department, Henan Province, Zhengzhou 450052, China
  • Received:2014-07-24 Online:2015-03-28 Published:2015-04-04

摘要:

目的:研究Wnt/β-catenin通路的关键分子β-catenin、c-myc和cyclinD1在NK/T细胞淋巴瘤(NKTCL)组织中的表达,阐明其与NKTCL患者临床特征的关系。方法:采用Real-time PCR法检测人NKTCL细胞株(SNK-6和YTS)及人正常NK细胞中Wnt/β-catenin信号通路相关基因β-catenin、c-myc和cyclinD1 mRNA的表达水平;采用免疫组织化学SP法检测NKTCL组织和反应性增生的淋巴组织中β-catenin、c-myc蛋白的表达阳性率,并分析蛋白表达与患者临床特征的关系;分析NKTCL患者中β-catenin和c-myc蛋白表达的相关性;Kaplan-Meier法分析β-catenin表达与NKTCL患者中位生存期的关系。结果:细胞株SNK-6和YTS中β-catenin、c-myc和cyclinD1 mRNA的表达阳性率明显高于正常NK细胞(P<0.05);NKTCL组织中β-catenin和c-myc蛋白表达阳性率(24%和56%)明显高于淋巴结反应性增生组织(0%和25%)(P<0.05);β-catenin表达与Ann Arbor分期有关联(P<0.05),c-myc表达与Ann Arbor分期和Ki-67水平有关联(P<0.05),NKTCL患者中β-catenin和c-myc表达呈正相关关系(r=0.770,P<0.05);β-catenin阳性表达患者中位生存期低于β-catenin阴性表达患者(P<0.05)。结论:Wnt/β-catenin信号通路中关键分子β-catenin、c-myc、cyclinD1在NKTCL组织中高表达,并且其表达水平与NKTCL患者临床分期有关联。

关键词: NK/T细胞淋巴瘤, Wnt/β-catenin信号通路, β-catenin, c-myc, cyclinD1

Abstract:

Objective To investigate the expressions of the key molecules β-catenin,c-myc,and cyclinD1 of the Wnt/β-catenin signal pathway in the NK/T-cell lymphoma (NKTCL) tissue, and to clarify their relationships with the clinicopathological features of the NKTCL patients.Methods Real-time PCR method was used to detect the expression levels of β-catenin,c-myc,and cyclinD1 mRNA in human NKTCL cell lines (SNK-6 and YTS) and normal NK cells;the positive expression rates of β-catenin and c-myc proteins in the NKTCL tissue and reactive hyperplasia of lymph node tissue were detected by immunohistochemistry SP method;the correlations among the expressions of β-catenin and c-myc proteins and the clinicopathological features of the NKTCL patients were analyzed;correlation analysis was used to analyze the relationship between the expressions of β-catenin and c-myc proteins of the NKTCL patients;Kaplan-Meier method was used to analyze the relationship between the expression of β-catenin and overall survival rate of the NKTCL patients.Results The positive expression rates of β-catenin,c-myc,and cyclinD1 mRNA in the SNK-6 cell line and YTS cell line were significantly higher than those in the normal NK cells(P<0.05);the expression β-catenin and c-myc proteins in NKTCL tissue(24% and 56%) were higher than those in reactive hyperplasia of lymph node tissue (0 and 25%)(P<0.05);the expression of β-catenin was correlated with Ann Arbor stage(P<0.05),and the expression of c-myc was correlated with Ann Arbor stage and Ki-67(P<0.05);there were positive correlations between the expression of β-catenin and the expression of c-myc in the NKTCL patients(r=0.770,P<0.05);the patients with β-catenin positive expression had a shorter overall survival time than the patients with negative expression (P<0.05).Conclusion The key molecules β-catenin,c-myc,and cyclinD1 of the Wnt/β-catenin signal pathway are highly expressed in the human NKTCL tissue,and they show positive correlations with Ann Arbor stage of the NKTCL patients.

Key words: NK/T cell lymphma, Wnt/β-catenin signal pathway, β-catenin, c-myc, cyclinD1

中图分类号: 

  • R733.4