神经干细胞移植联合促红细胞生成素对大鼠脊髓损伤的修复作用

doi:10.13481/j.1671-587x.20150225

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 333-337.doi: 10.13481/j.1671-587x.20150225

神经干细胞移植联合促红细胞生成素对大鼠脊髓损伤的修复作用

赵岩¹, 肖宇龙¹, 左媛¹, 王喜良¹, 霍洪军¹, 江建明², 闫慧博³

1. 内蒙古医科大学第二附属医院脊柱外科, 内蒙古呼和浩特 010030;
2. 南方医科大学附属南方医院脊柱外科, 广东广州 510515;
3. 南方医科大学第三附属医院脊柱外科, 广东广州 510630

收稿日期:2014-07-22 出版日期:2015-03-28 发布日期:2015-04-04
通讯作者: 肖宇龙, 教授, 硕士研究生导师(Tel:0471-6351230, E-mail:nmgxyl88@sohu.com) E-mail:nmgxyl88@sohu.com
作者简介:赵岩(1977-), 男, 内蒙古自治区赤峰市人, 副主任医师, 医学博士, 主要从事脊髓损伤的基础和临床研究。
基金资助:
内蒙古自治区科技厅自然科学基金资助课题(2011MS1139)

Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats

ZHAO Yan¹, XIAO Yulong¹, ZUO Yuan¹, WANG Xiliang¹, HUO Hongjun¹, JIANG Jianming², YAN Huibo³

1. Department of Spine Surgery, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, China;
2. Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;
3. Department of Spinal Surgery, Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, China

Received:2014-07-22 Online:2015-03-28 Published:2015-04-04

摘要/Abstract

摘要：

目的:观察神经干细胞(NSCs)联合促红细胞生成素(EPO)对横断性大鼠脊髓损伤的修复作用,为临床治疗脊髓损伤提供理论依据。方法:40只成年雌性Wistar大鼠建立大鼠T10全横断脊髓损伤模型,并随机分为对照组、NSCs组、EPO组和NSCs+EPO组,每组10只。术后8周采用NF-200免疫组织化学染色和免疫荧光染色对各组大鼠损伤区脊髓神经纤维再生情况进行形态学观察;应用BBB评分评估各组大鼠后肢运动功能恢复情况。结果:组织形态学观察,对照组大鼠横断处脊髓组织残端萎缩,脊髓白质和灰质间可见大量空洞形成,未见有明显的神经纤维再生;NSCs+EPO组大鼠横断处脊髓组织残端轻度萎缩,横断区可见大量呈杂乱、无序生长的神经纤维,可见有连续性神经纤维通过脊髓横断区,脊髓白质和灰质间可见少量空洞形成。NSCs+EPO组大鼠中,FITC共轭抗神经丝蛋白抗体NF-200标记的再生神经纤维在横断区头侧大量再生,呈无序状生长,并通过损伤区到达尾侧;NSCs组大鼠可见少量神经纤维再生,未通过损伤区到达尾侧。NSCs+EPO组大鼠后肢运动功能BBB评分,术后7 d内均高于其他各组(P<0.05);NSCs组大鼠后肢运动功能BBB评分术后7 d内均高于对照组和EPO组(P<0.05)。结论:NSCs移植联合腹腔注射EPO可有效促进大鼠损伤脊髓功能的恢复、轴突的存活和再生。

关键词: 神经干细胞, 促红细胞生成素, 细胞移植, 脊髓损伤

Abstract:

Objective To observe the repair effects of neural stem cells(NSCs) combined with erythropoietin (EPO) on transverse spinal cord injury in the adult rats,and to provide a theoretical basis for clinical treatment of spinal cord injury.Methods Forty Wistar rats were used to set up T10 spinal cord transaction models and were randomly divided into control group(n=10),NSCs group(n=10),EPO group(n=10), and NSCs+EPO group(n=10).NF-200 immunohistochemistry and immunofluorescence staining were used to detect the morphology of the regenerating nerve fibers of the rats in various groups 8 weeks after the operation;the BBB score was used to evaluate the restoration of motor function of the hind limbs of the rats in various groups.Results The results of histomorphology showed that the stump of the transected spinal cord tissue atrophied,and there were a large amount of the formation of hole between white matter and gray matter of spinal cord of the rats in control group.The regeneration of nerve fibers could not be seen clearly of the rats in control group.The stump of transected spinal cord tissue atrophied slightly of the rats in NSCs+EPO group,and a large amount of nerve fibers with messy and disorderly state were visible in the cross-sectional area,and there was a lot of continuous nerve fibers getting through the cross-sectional area,and a small number of the formation of hole between white matter and gray matter of spinal cord could be observed.In the experiment,a lot of FITC-conjugated anti-neurofilament antibody NF-200 labelled nerve fibers regenerated in the side of head of the transected area in NSCs+EPO group could be observed,which grew disorderly,and got through the tail side of the damage zone;However,there was a small number of the regeneration of nerve fibers in NSCs group,and these fibers didn't get through the damage zone.The BBB scores of motor function of the hind limbs of the rats in NSCs+EPO group were higher than those in other groups within 7 d after the operation(P<0.05),and the BBB scores of the rats in NSCs group were also higher than those in control group and EPO group(P<0.05).Conclusion NSCs transplantation combined with intraperitoneal injection of EPO could promote the recovery of the neurological function and benefit the survival and regeneration of injured axons after spinal cord injury in the adult rats.

Key words: neural stem cells, erythopoietin, cell transplantation, spinal cord injury

中图分类号:

R651.2

赵岩, 肖宇龙, 左媛, 王喜良, 霍洪军, 江建明, 闫慧博. 神经干细胞移植联合促红细胞生成素对大鼠脊髓损伤的修复作用[J]. 吉林大学学报(医学版), 2015, 41(02): 333-337.

ZHAO Yan, XIAO Yulong, ZUO Yuan, WANG Xiliang, HUO Hongjun, JIANG Jianming, YAN Huibo. Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats[J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 333-337.

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神经干细胞移植联合促红细胞生成素对大鼠脊髓损伤的修复作用

Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats

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