关键词: TCF7L2基因, 2型糖尿病, 宁夏回族, 单核苷酸多态性

Abstract:

Objective To investigate the association between rs290487 polymorphisms of transcription factor 7 like 2 (TCF7L2) gene and type 2 diabetes mellitus (T2DM) in Ningxia Hui population,and to clarify whether the TCF7L2 gene is the susceptible gene of T2DM.Methods Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was used to detect the distribution of the geotypic and allelic frequencies of rs290487 site of TCF7L2 gene of the objects,including 111 T2DM patients (T2DM group) and 109 healthy individuals (control group)in Ningxia Hui population. χ² test was used to compare the differences of the allelic frequency and genotypic frequcecy of the objects between two groups.Results Three genotyps TT,CT,and CC were found both in T2DM group and control group; the genotypic frenquencies of rs290487 site of TCF7L2 gene did not deviate from the Hardy-Weinberg equilibrium in two groups (P>0.05); there was no significant difference of the genotypic frequency of rs290487 site between two groups(χ²=5.370,P>0.05);there was no significant difference of the alleclic frequency of rs290487 site between two groups (χ²=0.034,P>0.05).Conclusion The polymorphisms of rs290487 site of TCF7L2 gene has no relationship with T2DM in Ningxia Hui population,and TCF7L2 gene may be not the susceptible gene of T2DM.

Key words: transcription factor 7 like 2, type 2 diabetes mellitus, Ningxia Hui population, single nucleotide polymorphisms