五倍子酸对人胃癌SGC-7901细胞迁移能力的影响

doi:10.13481/j.1671-587x.20170415

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 739-742.doi: 10.13481/j.1671-587x.20170415

五倍子酸对人胃癌SGC-7901细胞迁移能力的影响

王丽萍¹, 冯若¹, 邹立洋², 丁一¹

1. 郑州大学基础医学院组织学与胚胎学教研室, 河南郑州 450001;
2. 解放军第301医院输血科, 北京 100853

收稿日期:2016-04-21 出版日期:2017-07-28 发布日期:2017-08-01
通讯作者: 丁一,教授,硕士研究生导师(Tel:0371-67781959,E-mail:dingyi@zzu.edu.cn) E-mail:dingyi@zzu.edu.cn
作者简介:王丽萍(1977-),女,吉林省榆树市人,讲师,医学硕士,主要从事消化道肿瘤方面的研究。
基金资助:
河南省科技厅科技攻关项目资助课题（122102310233）；河南省卫计委科研项目资助课题（200803061）

Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells

WANG Liping¹, FENG Ruo¹, ZOU Liyang², DING Yi¹

1. Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China;
2. Department of Blood Transfusion Room, No. 301 Hospital of PLA, Beijing 100853, China

Received:2016-04-21 Online:2017-07-28 Published:2017-08-01

摘要/Abstract

摘要： 目的：探讨五倍子酸（GA）对人胃癌SGC-7901细胞转移的影响，阐明其作用机制。方法：培养人胃癌SGC-7901细胞，将其分为对照组及3.125、6.250、12.500、25.000、50.000和100.000 mg·L^-1GA组。MTT法检测各组SGC-7901细胞增殖抑制率，细胞划痕实验检测各组细胞转移能力，免疫细胞化学法检测各组SGC-7901细胞中血管内皮生长因子（VEGF）表达水平。结果：与对照组比较，不同剂量GA组SGC-7901细胞增殖抑制率明显升高，并呈剂量依赖性（F=59.451，P<0.01）。与对照组比较，不同剂量GA组SGC-7901细胞的划痕愈合率明显降低（P<0.01），12.500和25.000 mg·L^-1 GA组细胞中VEGF蛋白表达水平明显降低（P<0.05）。结论：GA通过抑制人胃癌SGC-7901细胞中VEGF蛋白的表达来抑制SGC-7901细胞增殖及迁移。

关键词: 五倍子酸, 细胞迁移, SGC-7901细胞, 血管内皮生长因子

Abstract: Objective: To study the influence of gallic acid (GA) on the migration of human gastric carcinoma SGC-7901 cells, and to explore its mechanism.Methods: The human gastric carcinoma SGC-7901 cells were cultured and divided into control group and 3.125, 6.250, 12.500, 25.000, 50.000,100.000 mg·L^-1 GA groups. The inhibitory rates of proliferation of SGC-7901 cells in various groups were examined by MTT assay; the migration abilities of SGC-7901 cells in various groups were measured with scratch assay; the expression levels of vascular of endothelial growth factor (VEGF) in various groups were detected by immunocytochemistry.Results: Compared with control group, the inhibitory rates of proliferation of SGC-7901 cells in different doses of GA groups were significantly increased in a dose-dependent manner(F=59.451,P<0.01). Compared with control group, the wound healing rates in different doses of GA groups were significantly decreased (P<0.01). Compared with control group, the expression levels of VEGF protein in 12.500 and 25.000 mg·L^-1 GA groups were decreased (P<0.05).Conclusion: GA could inhibit the proliferation and migration of SGC-7901 cells through down-regulating the expression levels of VEGF protein.

Key words: cell migration, vascular endothelial growth factor, gallic acid, SGC-7901 cells

中图分类号:

R285.5

王丽萍, 冯若, 邹立洋, 丁一. 五倍子酸对人胃癌SGC-7901细胞迁移能力的影响[J]. 吉林大学学报(医学版), 2017, 43(04): 739-742.

WANG Liping, FENG Ruo, ZOU Liyang, DING Yi. Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells[J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 739-742.

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五倍子酸对人胃癌SGC-7901细胞迁移能力的影响

Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells

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