人参活性糖肽的荧光标记鉴定及其在小鼠主要脏器组织中的分布

doi:10.13481/j.1671-587x.20200404

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (04): 687-692.doi: 10.13481/j.1671-587x.20200404

• 基础研究 • 上一篇

人参活性糖肽的荧光标记鉴定及其在小鼠主要脏器组织中的分布

房晓雪¹, 徐伟², 周婷婷³, 唐燕³, 王春驰³, 姜瑞芝³, 邱智东², 罗浩铭²

1. 长春中医药大学药学院中药药剂实验室, 吉林长春 130117;
2. 长春中医药大学药学院药物化学与中药化学教研室, 吉林长春 130117;
3. 吉林省吉测检测技术有限公司研发部, 吉林长春 130117

收稿日期:2019-09-03 发布日期:2020-08-20
通讯作者: 罗浩铭,副教授,博士研究生导师(Tel:0431-86172555,E-mail:luo.haoming@163.com) E-mail:luo.haoming@163.com
作者简介:房晓雪(1996-),女,吉林省德惠市人,在读医学硕士,主要从事药剂学方面的研究。
基金资助:
国家自然科学基金资助课题（81973468）；吉林省科技厅优秀青年人才基金项目资助课题（20180520056JH）；吉林省教育厅高校科技与社科"十三五"科研规划项目资助课题（JJKH20190474KJ）；吉林省中医药管理局中医药科技项目资助课题（2018DZ09）

Fluorescence labelingand identification of panax ginseng active glycopeptide and its distribution in main organ tissues of mice

FANG Xiaoxue¹, XU Wei², ZHOU Tingting³, TANG Yan³, WANG Chunchi³, JIANG Ruizhi³, QIU Zhidong², LUO Haoming²

1. Laboratory of Chinese Medicine, School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China;
2. Department of Pharmaceutical Chemistry and Traditional Chinese Medicine Chemistry, School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China;
3. Department Research and Development, Jilin Jice Inspection Technology Co., Lid., Changchun 130117, China

Received:2019-09-03 Published:2020-08-20

摘要/Abstract

摘要： 目的：对人参活性糖肽（PGG）进行荧光标记，观察其在小鼠主要脏器组织中的分布情况及其靶向趋势。方法：以异硫氰酸荧光素（FITC）为探针对PGG进行荧光标记，采用紫外分光光度法和红外分光光度法对PGG荧光标记物（PGG-FITC）进行结构分析，采用荧光分光光度法测定其荧光取代度。将78只小鼠随机分为13组，每组6只，其中6组小鼠注射PGG-FITC，6组小鼠注射FITC，1组小鼠作为空白对照组。给药后0.5、1.0、2.0、4.0、8.0和24.0 h，采集各组小鼠心、肝、脾、肺、肾、脑、肠和胃组织，采用荧光分光光度法测定小鼠主要脏器组织中PGG-FITC和FITC水平，并计算药时曲线下面积（AUC）和靶向效率（Te）。结果：PGG与FITC通过共价键连接，其荧光取代度为1.435%；与FITC组比较，PGG-FITC组小鼠主要脏器组织中的药物水平均明显升高（P<0.01），脑组织中药物水平升高更为明显；FITC组小鼠主要脏器组织中AUC值从高到低依次为肝、脑、胃、肾、肠、心、脾和肺组织，PGG-FITC组小鼠主要脏器组织中AUC值从高到低依次为脑、心、肝、肾、脾、胃、肺和肠组织。结论：利用FITC对PGG进行荧光标记，PGG经过尾静脉注射后主要富集在小鼠脑部，具有明显的脑靶向趋势。

关键词: 人参活性糖肽, 异硫氰酸荧光素, 荧光标记, 脑, 心脏, 肺

Abstract: Objective: To observe the distribution of panax ginseng active glycopeptide (PGG) in the main organ tissues of the mice and its targeting trend by fluorescence labeling. Methods: PGG was labeled with fluorescein isothiocyanate (FITC) as a probe. The structures of PGG fluorescence marker (PGG-FITC) were analyzed by ultraviolet spectrophotometry and infrared spectrophotometry. The fluorescence substitution degree of PGG-FITC was determined by fluorescence spectrophotometry. A total of 78 mice were randomly divided into 13 groups; the mice in 6 groups were injected with PGG-FITC, the mice in another 6 groups were injected with FITC, and the residual group was used as blank control. The heart, liver, spleen, lung, kidney, brain, intestine and stomach tissues of the mice in various groups were collected at 0.5, 1.0, 2.0, 4.0, 8.0 and 24.0 h after administration. The levels of PGG-FITC and FITC in the main organ tissues of the mice were determined by fluorescence spectrophotometry, and the area under concentration time curve (AUC) and targeting efficiency (Te) were calculated. Results: PGG and FITC were linked by covalent bond and the fluorescence substitution degree was 1.435%. Compared with FITC group, the drug levels in main organ tissues of the mice in PGG-FITC group were increased significantly (P<0.01), and the drug levels in brain tissue were increased more significantly. The AUC values in main organ tissues of the mice in FITC group from high to low were liver, brain, stomach, kidney, intestine, heart, spleen and lung tissues;and the AUC values in main organ tissues of the mice in PGG-FITC group from high to low were brain, heart, liver, kidney, spleen, stomach, lung and intestine tissues. Conclusion: PGG labeled by FITC is mainly enrich in the brain tissue of the mice after tail vein injection of PGG, and has a brain targeting trend.

Key words: panax ginseng active glycopeptide, fluorescein isothiocyanate, fluorescence labeling, brain, heart, lung

中图分类号:

R969.1

房晓雪, 徐伟, 周婷婷, 唐燕, 王春驰, 姜瑞芝, 邱智东, 罗浩铭. 人参活性糖肽的荧光标记鉴定及其在小鼠主要脏器组织中的分布[J]. 吉林大学学报(医学版), 2020, 46(04): 687-692.

FANG Xiaoxue, XU Wei, ZHOU Tingting, TANG Yan, WANG Chunchi, JIANG Ruizhi, QIU Zhidong, LUO Haoming. Fluorescence labelingand identification of panax ginseng active glycopeptide and its distribution in main organ tissues of mice[J]. Journal of Jilin University(Medicine Edition), 2020, 46(04): 687-692.

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人参活性糖肽的荧光标记鉴定及其在小鼠主要脏器组织中的分布

Fluorescence labelingand identification of panax ginseng active glycopeptide and its distribution in main organ tissues of mice

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