关键词: 生长抑素, 胰岛淀粉样多肽, 生后发育, 胃窦, 家兔

Abstract:

Abstract:Objective To study the localization and expressions of somatostatin(SS) and islet amyloid polypeptide(IAPP) in rabbit gastric antrum mucosa during the postnatal development period,and to explore the relationship between these hormones and digestive function.Methods Sixty rabbits were divided into 6 groups according to their age:5 d,15 d,25 d,35 d,60 d,and 90 d;10 rabbits in each group.The rabbits were excuted and their gastric antrum tissues were taken and embedded in routine paraffin,then the  serial sections were made．Immunohistochemical SABC staining and image analysis were performed to detect the expression and  localization of SS and IAPP immunoreactive positive cells.Results The expressions of SS and IAPP were found in the gastric antrum mucosa of rabbits at different postnatal development periods.In  5 d  and 15 d groups,a few SS positive cells were scattered into the mucosa epithelium and the staining  was weaker.From the 35th  day,the number of SS positive cells was ascended and obviously reached a peak in 60 d group （P<0.05）,and the number of SS positive cells in 90 d group was similar with that in 35 d group.The mean grey value of SS positive cells was decreased from the 35th day and reached the lowest in 60 d group （P<0.05）.The positive IAPP positive cells were predominantly located in the connective tissue of gastric antrum mucosa,occasionally in the gastric mucosal epithelium.The number of IAPP positive cells was gradually increased from the 5th day,especially in 60 d group（P<0.05）. The mean grey value of IAPP positive cells was decreased from the 35th day（P<0.05）.Conclusion The expressions of SS and IAPP in gastric antrum mucosa of rabbits change with the growth,which suggests that both of them should participate in the process of the digestive function by endocrine and paracrine ways．They play an important role in the growth repair and self-protection of the gastric mucosa.

Key words: somatostatin;islet amyloid polypeptide, postnatal development;gastric antrum, rabbit