关键词: 急性高原反应, 缺氧诱导因子1&alpha, 核转录因子kappaB p65亚基, 缺氧, cross-talk

Abstract:

Abstract:Objective To observe the  changes of expression levels of  hypoxia-inducible factor 1 alpha(HIF-1α)and nuclear factor kappa B(NF-κB) p65 (RelA) in
mononuclear cells  after ascenting of plateau  and their correlations with the incidence
 rate of acute mountain sickness(AMS),and to explore the role and mechanism of
 HIF-1α  and RelA in pathogenesis of AMS.Methods The soldiers’ venous blood before ascentting of plateau and 48 h after arriving Yushu were collected,and the p
rotein and mRNA in mononuclear cells were extracted.The expressions of HIF-1α and RelA mRNA were determined by RT-PCR.The expression of HIF-1α  protein was
determined by Western blotting method.The correlations between the expressions of HIF-1α  and RelA and pathogenesis of  AMS were analyzed with Spearman anal
ysis.Results Compared with before ascentting,after ascentting of plateau the expression levels of  HIF-1α mRNA,RelA mRNA and
 HIF-1α   protein  were increased significantly（P<0.01）.The expression of HIF-1α mRNA was positively correlated with the  RelA mRNA expression
（r=0.806,P<0.01）.At the same time,the expression  of HIF-1α  protein was positively  correlated with the pathogenesis of AMS（r= 0.875,P<0.01）;and the expression levels of HIF-1α  and RelA had no significant difference between women and men（P>0.05).Conclusion
The expression level of HIF-1α  protein is increased significantly after asentting of plateau,and has significantly positive correlation with the pathogenesis of AMS.The expression levels of HIF-1α  and RelA mRNA are significantly increased 48 h after ascentting of plateau,and they have positive correla
tion,prompting that the existence of cross-talk phenomenon between their signal transduction pathways.

Key words: acute mountain sickness, hypoxia-inducible factor 1 alpha , nuclear factor kappa B p65, hypoxia, cross-talk