吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

姜黄素对铁负载小鼠脑组织caspase-3、caspase-9和livin蛋白表达的影响及其对神经系统的保护作用

于嵩1,胡江平2    


  1. (1.辽宁中医药大学基础医学院解剖组胚教研室,辽宁 沈阳 110847;2.牡丹江医学院组织胚胎学教研室,黑龙江 牡丹江 157000)
  • 收稿日期:2013-04-09 出版日期:2013-09-28 发布日期:2013-07-28
  • 通讯作者: 于 嵩 E-mail:(Tel:024-31207087,E-mail:yuso1979@yahoo.com.cn)
  • 作者简介:于 嵩(1979-),女,黑龙江省五常市人,讲师,医学博士,主要从事中药成分对神经退行性疾病的治疗作用及其机制的研究。
  • 基金资助:

    国家自然科学基金资助课题(81203004)

Influence of curcumin in caspase-3,9 and livin expressions in brain tissue of iron load mice and its protective effect on nervous system

YU Song1,HU Jiang-ping2   

  1. (1.Department of Anatomy and Histology and Embryology,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;2.Department of Histology and Embryology,Mudanjiang Medical College,Mudanjiang 157000,China)
  • Received:2013-04-09 Online:2013-09-28 Published:2013-07-28

摘要:

目的:观察姜黄素对小鼠脑组织铁负载后caspase-3、caspase-9和livin蛋白表达的影响,探讨其对铁离子所诱导的中枢神经系统毒性作用的保护机制。方法:将30只CD1小鼠按双盲法随机分为生理盐水对照组、FeCl3 组和姜黄素干预组,每组10只。3组小鼠脑组织分别行尼氏染色观察海马神经元形态,免疫组织化学染色和Western blotting法检测小鼠脑组织caspase-3、caspase-9和livin蛋白表达强度和水平。结果:尼氏染色结果,与对照组比较,FeCl3组小鼠海马出现神经元丢失,明显细胞核浓缩和细胞核裂解消失现象;姜黄素干预组小鼠海马神经元萎缩和核裂解消失现象减少。免疫组织化学染色及Western blotting检测结果,与对照组比较,FeCl3组小鼠脑组织caspase-3、caspase-9蛋白表达水平明显升高,livin蛋白表达水平降低(P<0.05);姜黄素干预组小鼠脑组织,caspase-3和caspase-9蛋白表达水平降低,livin蛋白表达水平增强(P<0.05)。结论:姜黄素对铁离子所致小鼠神经元毒性具有明显抑制作用,其可能机制是抑制了凋亡通路中的caspase-3和caspase-9蛋白,同时激活了凋亡抑制因子livin蛋白。

关键词: 神经细胞, 毒性作用, 姜黄素, 铁离子

Abstract:

Abstract:Objective
To investigate  the influence of curcumin in caspase-3,9 and livin expressions in brain tissue of iron load mice ,and to exlore the protection mechanisms of curcumin on iron ion-induced toxic effects of central nervous system.Methods According to the double blind method,CD1 mice were randomly divided  into  control group,FeCl3 group and curcumin intervention group (n=10).The mouse brain tissues in three groups  were conducted with Nissl staining and  immunohistochemical staining, and the expressions of caspase-3,caspase-9 and livin were detected with Western blotting method.Results The Nissl staining results showed that compared with control group,the neurons of the mice in FeCl3 group were lost and existed  nuclear condensation and fragmentation in hippocampus,but they  were weaken after injection with curcumin.Compared with control group,the expression levels of caspase-3 and caspase-9 protein in brain tissue of mice were significantly increased and the expression levels of livin protein was decreased in  FeCl3 group(P<0.05).Compared with FeCl3 group,the expression levels of caspase-3 and caspase-9 protein were reduced and the livin protein expression level was increased in  curcumin group(P<0.05).Conclusion Curcumin can significantly inhibit the neurotoxicity of iron ions,the mechanism may be related to  inhibiting caspase-3 and caspase-9  in the apoptosis pathway and activating  the apoptosis-inhibited  factor  livin expression.

Key words: curcumin, iron ion, nerve cell, toxic actions

中图分类号: 

  • R285