苦碟子注射液对豚鼠噪声暴露后内耳功能恢复的影响

doi:吉林省科技厅资助课题 (20010534 )

苦碟子注射液对豚鼠噪声暴露后内耳功能恢复的影响

张岩，杜波，杜宝东^*

吉林大学第一医院耳鼻咽喉-头颈外科，吉林长春130021

收稿日期:2005-07-28 修回日期:1900-01-01 出版日期:2006-03-28 发布日期:2006-03-28
通讯作者: 杜宝东^*

Effect of Ixeris sonchifoila on inner ear functionof guinea pig after noise exposure

ZHANG Yan，DU Bo，DU Bao-dong^*

Department of Otorhinolaryngology and Head-Neck Surgery, First Hospital,Jilin University, Changchun 130021，China

Received:2005-07-28 Revised:1900-01-01 Online:2006-03-28 Published:2006-03-28
Contact: DU Bao-dong^*

摘要/Abstract

摘要： 目的：观察中药苦碟子注射液（Ixeris sonchifoila）对噪声暴露后内耳功能恢复的影响。方法：42只豚鼠随机分为2组，即110 dB噪声暴露组（n=21）和120 dB噪声暴露组（n=21），每组动物随机分为噪声暴露后给药组（n=7）、生理盐水组（n=7）和空白对照组（n=7）。噪声暴露前分别测量各组豚鼠听觉脑干诱发电位(ABR)听力阈值，持续噪声暴露3 h后，给药组每日1次腹腔注射中药苦碟子注射液15.7 mL·kg^-1·d^-1，连续14 d，生理盐水组腹腔注射同等量生理盐水，空白对照组不给予处置。噪声暴露前、噪暴露后即刻及噪声暴露后7和14 d分别测量各组豚鼠ABR听力阈值，并对120 dB噪声暴露组进行耳蜗铺片观察毛细胞形态改变。结果：110和120 dB噪声暴露2组中，7和14 d给药组ABR阈移均小于生理盐水注射组和空白对照组，与后2组比较差异均具有显著性（P<0.01）。110和120 dB噪声暴露后，给药组14 d ABR阈移均小于7 d，两者比较差异具有显著性（P<0.01）；110 dB给药组14 d阈移小于120 dB给药组，两者比较差异具有显著性（P<0.01）；120 dB给药组与生理盐水组和空白对照组比较，毛细胞形态保持完好。结论：中药苦碟子注射液能够促进噪声作用后的耳蜗毛细胞损伤的修复及内耳听力功能恢复；内耳功能的恢复程度与噪声的强度及给药时间有关。

关键词: 菊科, 阈移, 耳蜗, 毛细胞, 测听法, 诱发反应

Abstract: Objective To observe the effect of Ixeris sonchifoila, a kind of Chinese traditional medicine on noise-induced hearing loss. Methods Fourty-two guinea pigs were divided into two groups. The animals were exposed under 110 dB (n=21) and 120 dB (n=21) noise, separately. Each group was divided into medicine group (n=7), saline group(n=7) and control group (n=7). Ixeris sonchifoila was used in the medicine group and saline in the saline group immediately after the noise exposure by abdomen injection. The threshold was recorded 7 and 14 d later by ABR. Cochlea patch was performed to find hair cell loss. Results By using Ixeris sonchifoila, the threshold shift was significantly reduced (P<0.01) and the hair cells had less injuries in both of 110 and 120 dB groups, the threshold shift of 14 d later was less than that of 7 d later in medicine groups (P<0.01). Conclusion Ixeris sonchifoila can reduce the hair cell injury induced by noise and improve hearing, the effect depends on the degree of noise and the time of Ixeris sonchifoila injection.

Key words: compositae, threshold shift, cochlea, hair cells, audiometry, evoked response

中图分类号:

TB53

张岩，杜波，杜宝东. 苦碟子注射液对豚鼠噪声暴露后内耳功能恢复的影响[J]. J4, 2006, 32(2): 261-263.

ZHANG Yan，DU Bo，DU Bao-dong. Effect of Ixeris sonchifoila on inner ear functionof guinea pig after noise exposure[J]. J4, 2006, 32(2): 261-263.

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