关键词: 骨髓细胞, 粒细胞集落刺激因子, 白细胞介素11

Abstract: Objective To investigate the effect of As₂O₃on normal mouse bone marrow stromal cells(BMSCs). Methods BMSCs and colony-forming unit-granulocyte macrophage(CFU-GM) were cultured in vitro to observe the quantitative changes of colony-forming unit-fibroblast (CFU-F) and CFU-GM after BMSCs were treated with different concentrations of As₂O₃respectively;the quantitative changes of CFU-GM supported by BMSCs and granulocyte colony stimulating factor(G-CSF) and IL-11 secreted by BMSCs were also observed after the progenitor cells supported by BMSCs were treated with As₂O₃ for 3，5 and 7 d，respectively. Results The productions of CFU-F and CFU-GM supported by BMSCs were decreased obviously when treated with 7 μmol·L^-1 As₂O₃, also the levels of G-CSF and IL-11 secreted by BMSC(P<0.05). The levels of G-CSF and IL-11 secreted by BMSCs were increased obviously if treated with 3 μmol·L^-1 As₂O₃ continuously for 3 or 5 d, whereas without increasing obv iously when treated with 3 μmol·L^-1 As₂O₃ continuously for 7 d and later (P<0.05). Conclusion Lower concentration of As₂O₃has not significant inhibitory effects on clone forming of BMSCs and the productions of CFU-GM supported by BMSCs, but stimulates BMSCs to secret G-CSF and IL-11. The concentration of As₂O₃ causing the max level of G-CSF and IL-11 is 3 μmol·L ^-1.The administration time when achieved the max levels of G-CSF and IL-11 is 5 d. The higher concentration of As₂O₃and the elongation of the administration time can decrease the secretion of G-CSF and IL-11.

Key words: sub>O_3<, sub>, bone marrow cells, granulocyte colony stimulating factor, interleukin-11