TGF-β1对大鼠肾小球系膜细胞PAI-1表达的影响

doi:吉林省科技厅资助课题(200505156)

TGF-β1对大鼠肾小球系膜细胞PAI-1表达的影响

姚海舟¹，李洪岩²，张英³，周希君³，李才⁴，孔祥波^1*

1. 吉林大学中日联谊医院泌尿外科，吉林长春130033; 2. 吉林大学基础医学院病理生理学教研室，吉林长春130021；3. 吉林大学再生医学科学研究所生物化学研究室，吉林长春130021;4. 吉林大学再生医学科学研究所

收稿日期:2005-10-30 修回日期:1900-01-01 出版日期:2006-05-28 发布日期:2006-05-28
通讯作者: 孔祥波

Effects of TGF-β1 on expression of PAI-1 in rat mes angial cells

YAO Hai-zhou¹,LI Hong-yan²，ZHANG Ying³, ZHOU Xi-jun³, LI Cai⁴, KONG Xiang-bo^1*

1. Department of Urology, China-Japan Union Hospital, Jilin University, Changchun 130033, China;2. Department of Pathophysiology, School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3. Department of Biochemistry, Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China;4. Department of Pathology, Institute of Frontier Medical Sciences,Jilin University, Changchun 130021, China

Received:2005-10-30 Revised:1900-01-01 Online:2006-05-28 Published:2006-05-28
Contact: KONG Xiang-bo

摘要/Abstract

摘要： 目的：观察不同浓度重组人转化生长因子-β1（TGF-β1）对大鼠肾系膜细胞增殖情况和纤溶酶原激活物(PAI-1)抑制物表达水平的影响。方法：采用MTT和流式细胞仪检测细胞增殖，Western blotting和RT-PCR法检测PAI-1的表达水平。结果：与对照组相比，0.1和0.5 μg·L^-1 TGF-β1诱导肾小球系膜细胞增殖，增殖率分别为(146.6±10.2)%和(134.6±14.5)%，PAI-1 mRNA和蛋白表达增强；而5和25 μg·L^-1 TGF-β1诱导的细胞增殖率为(75.2±13.2)%和(67.5±11.1)%，PAI-1 mRNA和蛋白表达无明显变化。结论：低剂量TGF-β1促进大鼠系膜细胞增殖、诱导PAI-1表达；高剂量TGF-β1则抑制系膜细胞增殖。

关键词: 转化生长因子β, 纤溶酶原激活抑制物1

Abstract: Objective To study the effects of transforming growth factor-β1 (TGF-β1) on mesangial cells growth and expression of plasminogen activator inhibitor-1(PAI-1) in cultured rat mesangial cells stimulated by different levels of human recombinant TGF-β1. Methods The proliferation of mesangial cells was determined with MTT assay and FACS analysis. The levels of PAI-1 protein and mRNA were analyzed with Western blotting analysis and RT-PCR respectively. Results Compared with control group, 0.1 and 0.5 μg·L^-1 TGF-β1 stimulated mesangial cells proliferation, the proliferation rate of mesangial cell was (146.6±10.2)% and (134.6±14.5)% respectively, and the expressions of PAI-1 mRNA and protein also increased (P<0.05). The proliferation rate was （75.2±13.2%） and （67.5±11.1）% in the group treated with 5 and 25 μg·L^-1 TGF-β1, the expressions of PAI-1 mRNA and protein were constant. Conclusion Low level of TGF-β1 can trigger the proliferation of rat mesangial cells, induce the expression of PAI-1, and high level of TGF-β1 can inhibit the proliferation of mesangial cells.

Key words: transforming growth factor beta, plasminogen activator inhibitor 1

中图分类号:

姚海舟，李洪岩，张英，周希君，李才，孔祥波. TGF-β1对大鼠肾小球系膜细胞PAI-1表达的影响[J]. J4, 2006, 32(3): 425-427.

YAO Hai-zhou,LI Hong-yan，ZHANG Ying, ZHOU Xi-jun, LI Cai, KONG Xiang-bo. Effects of TGF-β1 on expression of PAI-1 in rat mes angial cells[J]. J4, 2006, 32(3): 425-427.

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