中草药仙鹤草对雄性小鼠生殖细胞的遗传毒性

doi:吉林省科技厅科技发展计划项目资助课题(200

中草药仙鹤草对雄性小鼠生殖细胞的遗传毒性

庞慧民¹, 朱玉琢^1*，乔萍²，温得中¹

1. 吉林大学基础医学院医学遗传学教研室,吉林长春130021；2. 吉林大学基础医学院药理学教研室，吉林长春130021

收稿日期:2005-10-20 修回日期:1900-01-01 出版日期:2006-05-28 发布日期:2006-05-28
通讯作者: 朱玉琢

Genetic toxicity of Agrimonia Pilosa Ledeb in male mouse genital cells

PANG Hui-min¹，ZHU Yu-zhuo^1*，QIAO Ping²,WEN De-zhong¹

1. Department of Medical Genetics,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2. Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China 

Received:2005-10-20 Revised:1900-01-01 Online:2006-05-28 Published:2006-05-28
Contact: ZHU Yu-zhuo

摘要/Abstract

摘要： 目的:观察中草药仙鹤草对雄性小鼠生殖细胞的遗传毒性。方法:将30只小鼠随机分为6组（每组5只）：阴性对照组（NS）、丝裂霉素C或甲基磺酸甲酯（MMC或MMS）组及仙鹤草诱变组(1.0、2.0、4.0和8.0 g·kg^-1)。采用小鼠精子畸形实验、精原细胞姐妹染色单体互换实验和小鼠精子非程序DNA合成实验,研究仙鹤草对生殖细胞的遗传毒性。结果:仙鹤草各剂量组所诱导的小鼠精子畸形频率和精原细胞姐妹染色单体互换频率与正常对照组比较,差异无显著性(P>0.05),仙鹤草不能诱导小鼠精子非程序DNA合成。结论:在本实验所使用的剂量范围内仙鹤草对雄性小鼠生殖细胞无遗传毒性。   

关键词: 诱变试验, 生殖细胞

Abstract: Objective To examine the genetic toxicity of Agrimonia Pilosa Ledeb on male mouse reproductive cells.Methods Sperm abnormality test, sister chromatid exchange (SCE) test in mouse spermatogonia and unscheduled DNA synthesis (UDS) test in mouse sperms were used.Thirty mice were divided into six groups (5 mice per group): negative control（NS）, Mitomycin C (MMC) or methyl methyl sulfonate (MMS) group，and Agrimonia Pilosa Ledeb groups(1.0,2.0,4.0 and 8.0 g·kg^-1). Results Sperm abnormality or SCE had no significant differences between test groups and control group (P>0.05),indicating that Agrimonia Pilosa Ledeb could not induce any UDS in mouse sperms. Conclusion Agrimonia Pilosa Ledeb has no genetic toxicity in male mouse reproductive cells at the range of doses that are applied under the experimental conditions.

Key words: mutagenesis test, germ cells

中图分类号:

R285.5

庞慧民, 朱玉琢，乔萍，温得中. 中草药仙鹤草对雄性小鼠生殖细胞的遗传毒性[J]. J4, 2006, 32(3): 445-447.

PANG Hui-min，ZHU Yu-zhuo，QIAO Ping,WEN De-zhong. Genetic toxicity of Agrimonia Pilosa Ledeb in male mouse genital cells[J]. J4, 2006, 32(3): 445-447.

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