胰激肽原酶对自发性高血压大鼠心肌纤维化及NO含量的影响

doi:吉林省科技厅资助课题（200505199）

胰激肽原酶对自发性高血压大鼠心肌纤维化及NO含量的影响

付军, 冷吉燕，杜萍，李颖新，刘补尚

吉林大学第一医院干部病房，吉林长春130021

收稿日期:2006-03-09 修回日期:1900-01-01 出版日期:2006-09-28 发布日期:2006-09-28

Effects of pancreatic kininogenase on myocardical fibrosis and serum nitric oxide level in spontaneously hypertensive rats

FU Jun,LENG Ji-yan,DU Ping,LI Ying-xin,LIU Bu-shang

Department of Cadre Ward，First Hospital,Jinlin University, Changchun 130021, China

Received:2006-03-09 Revised:1900-01-01 Online:2006-09-28 Published:2006-09-28

摘要/Abstract

摘要： 目的：探讨胰激肽原酶对自发性高血压大鼠（SHR）血压、心肌纤维化及血清一氧化氮（NO）含量的影响。方法：雄性15周龄SHR 24只，随机分成SHR组、胰激肽原酶组和卡托普利组，每组8只，另取8只雄性15周龄Wistar大鼠作为正常血压对照组。胰激肽原酶组腹腔注射（7.2 U•kg^-1•d^-1），卡托普利组灌胃给药（10 mg•kg^-1•d^-1），SHR组和正常血压对照组给0.9% NaCl溶液腹腔注射（2 mL•kg^-1•d^-1），各组均连续4周给药。实验4周后行颈动脉插管测量血压，然后处死动物，测量各组左心室重量指数（LVMI）、心肌胶原体积比例（CVF）、心肌血管周围胶原与管腔面积的比例（PVCA）及血清NO含量。结果：SHR组SBP、LVMI、CVF、PVCA增高，血清NO含量下降，与正常血压对照组比较差异均具有显著性（P<0.05）。用药4周后，胰激肽原酶组SBP、LVMI、CVF及PVCA均较SHR组显著下降，两组比较差异均具有显著性（P<0.05）；胰激肽原酶组NO含量明显升高且接近正常血压对照组水平；胰激肽原酶组除SBP外的各项指标与卡托普利组比较差异均无显著性（P>0.05）。结论：胰激肽原酶具有降低SHR血压和逆转心肌纤维化的作用，其机制可能与增加NO含量有关。

关键词: 治疗应用, 一氧化氮, 心内膜心肌纤维化, 大鼠, 近交SHR

Abstract: Objective To investigate the effects of pancreatic kininogenase on pressure,myocardical fibrosis and serum nitric oxide (NO) level in spontaneously hypertensive rats (SHR). Methods Twenty-four (fifteen weeks) male SHR were randomly divided into three groups:SHR group,pancreatic kininogenase group and captopril group（n=8 ），8 male Wistar kyoto rats with normal blood pressure were considered as control group. Pancreatic kininogenase was given by peritoneal injection (7.2 U•kg^-1•d^-1), captopril was given by intragast ric administration（10 mg•kg^-1•d^-1）, the rats in SHR group and rats with normol blood pressure in control group were treated with 0.9% NaCl（2 mL•kg^-1•d^-1）administered through peritoneal injection. After four-week experiment, the pressure was measured in rats througth carotid artery,then the rats were sacrificed , and LVMI,CVF,PVCA and serum NO level were measured. Results In SHR group, SBP,LVMI,CVF and PVCA were higher, serum NO level was decreased obviously than those in control group (P<0.05). After treatment of pancreatic kininogenase,the serum NO level raised obviously,other indexes obviously reduced than those in SHR group (P<0.05),but except SBP,there were no significant difference compared with captopril group (P>0.05). Conclusion Pancreatic kininogenase can obviously reduce the blood pressure and reverse myocardial fibrosis,its mechanism may be concerned with the increasing of NO level in SHR.

Key words: therapeutical use, nitric oxide, endomyocardial fibrosis, rats, inbred SHR

中图分类号:

R544.1

付军, 冷吉燕，杜萍，李颖新，刘补尚. 胰激肽原酶对自发性高血压大鼠心肌纤维化及NO含量的影响[J]. J4, 2006, 32(5): 826-828.

FU Jun,LENG Ji-yan,DU Ping,LI Ying-xin,LIU Bu-shang. Effects of pancreatic kininogenase on myocardical fibrosis and serum nitric oxide level in spontaneously hypertensive rats[J]. J4, 2006, 32(5): 826-828.

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