关键词: 羟基磷灰石, 生物可吸收材料, 生物相容性

Abstract: To investigate the biocompatibility of poly(L-lactide)/poly(L-lactide) surface grafted hydroxyapatite composite(PLLA/PLLA-gHAP) as a new biodegradable material, and provide a biological evidence for the application of the material. Methods　The L929 cell bioactivities in PLLA/PLLA-gHAP group, PLLA group, negative control group(DMEM culture media),positive control group（DMEM contain phenol solution）were determined by MTT assay after coculture for 24,48 and 72 h. L929 cells were cultivated on cover glide coated with PLLA/PLLA-gHAP composite for 1, 2 and 3 d, phase contrast microscope was used to observe the morphological changes of the cells. The leaching liquor of the material was injected into mice to test the acute toxicity and genetic toxicity of it. The PLLA/PLLA-gHAP plates were implanted subcutaneously into rabbit, and the anatomical, pathological and hematological changes were examined after 2 weeks, 3 months and 6 months. Results There was no significant difference in cytotoxicity between the PLLA/PLL　A-gHAP goup and negative control group, and the L929 cells grew well on the cover glide which goated with PLLA/PLLA-gHAP composite. No any acute toxicity or genetic toxicity was found in mice. There was only mild inflammatory reaction and thin fibrosis membrane in the early days after PLLA/PLLA-gHAP plate　 Implantation, the fibrosis membrane was nearly disappeared and most of the imflammatory reaction recovered 6 months later. Conclusion　The new bioabsorbable material, PLLA/PLLA-gHAP composite, has good biocompatibility .

Key words: hydroxyapatite, biodegradable material, biocompatibility