人胶质瘤细胞的侵袭能力和胶原溶解作用的体外研究

doi:吉林省科技厅科技发展计划重大项目资助课题

• 专题研究-体质人类学 • 上一篇下一篇

人胶质瘤细胞的侵袭能力和胶原溶解作用的体外研究

李蕴潜¹，赵丽艳²，李才³

1. 吉林大学第一医院神经外科，吉林长春 130021；2. 吉林大学第二医院检验科，吉林长春 130041；3. 吉林大学再生医学科学研究所病理研究室，吉林长春 130021

收稿日期:2006-03-29 修回日期:1900-01-01 出版日期:2007-01-28 发布日期:2007-01-28
通讯作者: 李才

Invasion ability and collagenolytic activity of human glioma cells in vitro

LI Yun-qian¹, ZHAO Li-yan², LI Cai³

1. Department of Neurosurgery, First Hospital, Jilin University, Changchun 130021, China; 2. Department of Laboratory, Second Hospital, Jilin University, Changchun 130041, China; 3. Department of Pathology, Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China

Received:2006-03-29 Revised:1900-01-01 Online:2007-01-28 Published:2007-01-28
Contact: LI Cai

摘要/Abstract

摘要： 目的：观察人胶质瘤细胞的体外侵袭能力及对胶原的溶解作用。方法：在体外用Boyden小室侵袭实验评价人恶性胶质瘤细胞株U87MG穿过Matrigel基膜胶的迁移能力；用琼脂-明胶凝胶观察U87MG细胞条件培养基对基质胶原溶解的影响。结果：U87MG胶质瘤细胞穿过Matrigel膜的细胞数明显多于加入EDTA或加入抗MMP-2抗体的U87MG胶质瘤细胞的穿膜细胞数（P<0.01），而后两者穿膜细胞数之间的差异无显著性（P>0.05），EDTA或抗MMP-2抗体对U87MG细胞的穿膜细胞数有明显的抑制作用，抑制率分别为79.2%和77.1%。U87MG细胞条件培养液能在凝胶孔周围引起明显增大的透明环，用EDTA或抗MMP-2抗体抑制MMP-2酶活性可明显减少透明环的形成。结论：U87MG胶质瘤细胞的侵袭能力和胶原降解作用取决于MMP-2，提示MMP-2在胶质瘤侵袭生长中起重要作用。

关键词: 侵袭能力, 胶原降解, 明胶酶A

Abstract: To investigate the invasion ability and collagenolytic activity of human glioma cells in vitro. Methods Boyden chamber invasion assay was employed to evaluate the cell migration ability of human malignant glioma cell line U87MG in vitro and the effect of conditioned medium of U87MG cells on matrix collagenolytic activity was tested by agar-gelatin gel. Results The number of U87MG glioma cells migrating through the Matrigel-coated membrane was more than that of addition of EDTA or anti-MMP-2 antibody in U87MG glioma cells (P<0.01), and the difference between the numbers of the migrated cells in the latter two was not significant (P>0.05). EDTA or anti-MMP-2 antibody markedly inhibited the number of the migrated cells, the inhibitory rates were 79.2% and 77.1%, respectively. The conditioned medium collected from the U87MG cells showed an increase in the transparent ring area on agar-gelatin gel. Inhibition of MMP-2 enzymatic activity by EDTA or anti-MMP-2 antibody reduced the transparent ring area on agar-gelatin gel. Conclusion Both invasion ability and collagenolytic activity of U87MG cells are depended on MMP-2, suggesting that MMP-2 plays an important role in glioma invasiveness.

Key words: invasion ability, collagen degradation, gelatinase A

中图分类号:

R739.91

李蕴潜，赵丽艳，李才. 人胶质瘤细胞的侵袭能力和胶原溶解作用的体外研究[J]. J4, 2007, 33(1): 84-87.

LI Yun-qian, ZHAO Li-yan, LI Cai. Invasion ability and collagenolytic activity of human glioma cells in vitro[J]. J4, 2007, 33(1): 84-87.

[1]	王葆春\|孙杨柳\|宫晓光\|费瑞 . RNA干扰沉默STAT3基因表达对人胰腺癌细胞SW1990侵袭能力的影响[J]. J4, 2011, 37(4): 636-640.
[2]	刘淑霞，郭惠芳，郝军，张玉军，唐丽娟，陈宁，段惠军. INF-γ对体外培养的系膜细胞HMGB1和MMP-2表达的影响[J]. J4, 2009, 35(1): 47-50.
[3]	潘颖，朱丹，司乃文，张文颖. 卵巢上皮性癌组织RhoC和MMP-2蛋白的表达及其临床意义[J]. J4, 2008, 34(6): 1042-1045.
[4]	马岩，李晓林，陆艳娟,李晓梅，刘坤，高航，张海英，马颖哲，韩智冲，张蒙. 黄蘑碱溶性多糖对荷瘤小鼠MMPs表达的调节及其抗肿瘤作用机制[J]. J4, 2008, 34(4): 605-609.
[5]	罗萍，黄兰，卢雪红，张冬梅. 慢性肾小球肾炎患者血清MMP-2表达及临床意义[J]. J4, 2007, 33(4): 741-743.
[6]	郝冰，孟晓萍，杨东华，王莉，郭昊. 普罗布可对冠心病患者血清中MMP-2的抑制作用[J]. J4, 2007, 33(3): 559-561.
[7]	盛辉，苑春莉，周洪澜，王医术. MMP-2和TIMP-2在肺癌组织中的表达及其与微血管密度的关系[J]. J4, 2006, 32(6): 1059-1062.
[8]	梅红，郭玉芳，郑贤红，王心蕊，张丽红，朱桂彬，王建民，李一雷. 整合素α_Vβ₃在细胞外基质调控人黑色素瘤MMP-2表达和活化中的作用[J]. J4, 2006, 32(2): 182-185.
[9]	邢王月，迟宝荣，李淑梅，景瑕斌，孟晓萍. 聚乳酸-聚乙醇酸-聚乙二醇血管支架置入对兔动脉粥样硬化模型血清基质金属蛋白酶2，9的影响[J]. J4, 2006, 32(2): 257-260.
[10]	郭玉芳，梅红，张丽红，刘巍，郑贤红，李一雷. 人乳腺癌组织活化型MMP-2的分布及意义[J]. J4, 2006, 32(1): 103-3.
[11]	张志超,孙亚欣,贾明库. 基质金属蛋白酶含量及表达与肝细胞癌侵袭转移的关系[J]. J4, 2006, 32(1): 116-3.
[12]	张宇靖，谷丽萍，华莹，张首杰. 基质金属蛋白酶-2及其组织抑制剂和微血管密度在子宫内膜癌中的表达[J]. J4, 2006, 32(1): 106-4.
[13]	陈永胜，潘留兰，金珍婧，杨力，迟宝荣, 李静. MMP-2、MMP-9和TIMP-1在胃癌组织中的表达及其与临床病理特点和预后的关系[J]. J4, 2005, 31(6): 927-930.
[14]	马岩，马颖哲，张家颖，李晓梅. 黄蘑多糖Fb对小鼠免疫功能的调节作用[J]. J4, 2005, 31(5): 692-695.
[15]	李蕴潜，谭岩，韩雪梅，李才. 人胶质瘤MMP-2及TIMP-2 mRNA表达和酶活性的改变及其意义[J]. J4, 2005, 31(3): 432-435.

人胶质瘤细胞的侵袭能力和胶原溶解作用的体外研究

Invasion ability and collagenolytic activity of human glioma cells in vitro

RichHTML

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0