聚乳酸-聚羟基乙酸植入大鼠拔牙创对骨愈合的影响

doi:国家自然科学基金资助课题(30471893) ；吉

聚乳酸-聚羟基乙酸植入大鼠拔牙创对骨愈合的影响

吴哲¹，刘畅²，郭雷¹，陈远³，孙宏晨⁴

1.吉林大学口腔医院修复科，吉林长春 130041；2.吉林大学口腔医院正畸科，吉林长春 130041；3.吉林省四平市中心医院骨科，吉林四平 136000；4.吉林大学口腔医院病理科，吉林长春 130041

收稿日期:2006-09-13 修回日期:1900-01-01 出版日期:2007-03-28 发布日期:2007-03-28
通讯作者: 孙宏晨

Effects of filling extraction socket with polylactic acid/polyglycolic acid on bone healing in rats

WU Zhe¹, LIU Chang²，GUO Lei¹， CHEN Yuan³, SUN Hong-chen⁴

1.Department of Prothodontics, Stomatology Hospital, Jilin University, Changchun 130041, China; 2. Department of Orthodontics,Stomatology Hospital,Jilin University, Changchun 130041,China;3.Department of Orthopedics,Central Hospital of Siping,Jilin Province, Siping 136000,China;4. Department of Pathology, Stomatology Hospital, Jilin University, Changchun 130041, China

Received:2006-09-13 Revised:1900-01-01 Online:2007-03-28 Published:2007-03-28
Contact: SUN Hong-chen

摘要/Abstract

摘要： 目的探讨多孔聚乳酸-聚羟基乙酸共聚物（PLGA）植入大鼠拔牙创对牙槽骨再生的影响。方法：选用健康雄性Wistar大鼠60只，随机分为实验组30只和对照组30只，拔除右下颌中切牙后，实验组即刻植入PLGA支架材料，自然愈合拔牙创作为对照组，于术后1、2、4、8和12周分别处死大鼠。用软X线及组织形态学方法评价大鼠下颌切牙拔牙窝的愈合情况。结果：术后4、8周与对照组相比，实验组剩余牙槽嵴长度明显降低（P<0.05）；术后 2、4和8周实验组剩余牙槽嵴的相对宽度高于对照组（P<0.05）。组织形态学显示，术后8周，实验组拔牙窝内可见极少量的未降解的支架材料，对照组拔牙窝内充满新生骨，但有间隙存在；术后12周，支架材料完全降解，实验组和对照组拔牙窝内均可见编织状骨，实验组骨沉积线明显。结论： PLGA生物材料在不影响牙槽窝骨组织愈合过程的同时，能有效地保持剩余牙槽嵴的长度和宽度。该材料不但具有良好的生物相容性和稳定性，而且具有骨引导的效应。

关键词: 牙槽窝, 聚乳酸-聚羟基乙酸 , 骨再生

Abstract: To observe the effects of porous polylactic acid/polyglycolic acid copolymer (PLGA) filling into extraction socket on the regeneration of alveolar bone in rats. Methods Sixty male Wistar rats were divided into experimental group and control group (n=30). PLGA scaffold was immediately implanted in the mandibular incisor root sockets after removal of incisor teeth. Soft X-ray photography, structural observation, light microscopy were used to evaluate the effects of composite on bone healing in a rat tooth extraction socket. Results The relative lengths of residual alveolar ridge in the experimental group 4,8 weeks after operation were shorter than that in the control group，and there were significant differences between the experimental group and the control group (P<0.05)．The relative width of residual alveolar ridge in the experimental group 2,4,8 weeks after operation were higher than that in the control group，and there were significant differences between the experimental group and the control group (P<0.05)．Histologic examination on the 8th week after operation showed that there was little undegradable scaffold in the experimental group, while the tooth socket in the control group was filled with new borne bone, and there was space between the new bone; the materials in the experimental group was degraded completely on the 12th week after operation, the woven bone was seen both in the experimental group and the control group. Conclusion The implantation of a degradable hydrophobic polymer does not significantly alter the procession of bone healing, and it can preserve the length and width of residual ridge. The new material is of good biocompatibility, biodegradability, stability, and osteoconduction.

Key words: tooth socket, porous polylactic acid, polyglycolic acid copolymer, bone remodeling

中图分类号:

R-332

吴哲，刘畅，郭雷，陈远，孙宏晨. 聚乳酸-聚羟基乙酸植入大鼠拔牙创对骨愈合的影响[J]. J4, 2007, 33(2): 234-236.

WU Zhe, LIU Chang，GUO Lei， CHEN Yuan, SUN Hong-chen. Effects of filling extraction socket with polylactic acid/polyglycolic acid on bone healing in rats[J]. J4, 2007, 33(2): 234-236.

[1]	焦鹏, 陈飞, 金权, 陈楠楠, 张莉, 马宁. 重度牙周炎致牙齿松动脱落患者前牙美学区综合治疗1例报告及文献复习[J]. 吉林大学学报(医学版), 2018, 44(02): 421-424.
[2]	田大川, 李海乐, 肖大伟, 周山健, 苏永蔚, 刘丹平, 綦惠. 联合应用软骨再生支架与突变型HIF-1α修饰BMSCs分泌的外泌体对晚期软骨缺损修复的促进作用[J]. 吉林大学学报(医学版), 2018, 44(02): 216-222.
[3]	陈飞, 史金先, 焦鹏, 金权, 许立硕, 张莉, 马宁. 不良修复体致前牙区残根残冠患者的牙周手术治疗1例报告及文献复习[J]. 吉林大学学报(医学版), 2018, 44(01): 170-174.
[4]	平飞云\|苗卓伟\|王新木\|董研. 壳聚糖在引导骨再生膜中的应用[J]. J4, 2012, 38(4): 813-816.
[5]	高心，何钟勤，王成坤，张志民，王志刚，钟丞，欧阳喈. 骨缺损修复材料煅烧牛骨粉的理化特性[J]. J4, 2009, 35(1): 138-141.