白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶活性与其毒力的关系

doi:吉林省科技厅资助课题 （970565-3）

白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶活性与其毒力的关系

曹岩¹，张巨²，沙春蕊³，郑永晨¹，刘永红⁴

1. 吉林大学第二医院中心实验室，吉林长春130041; 2 .吉林大学中日联谊医院手外科，吉林长春130033; 3.吉林大学第二医院麻醉科，吉林长春 130041; 4.吉林省肿瘤医院电诊室，吉林长春130012

收稿日期:2006-08-18 修回日期:1900-01-01 出版日期:2007-05-28 发布日期:2007-05-28
通讯作者: 郑永晨

Relationship between activity and virulence of secretory acid proteinaseand extracellular phosphlipase of Candida albicans

CAO Yan¹， ZHANG Ju²， SHA Chun-rui³， ZHENG Yong-chen¹， LIU Yong-hong⁴

1.Central Laboratory，Second Hospital，Jilin University,Changchun 130041，China；2.Department of Hand Surgery，China-Japan Union Hospital, Jilin University，Changchun 130033，China；3.Department of Anesthesiology，Second Hospital，Jilin University,Changchun 130041，China; 4.Department of Electrodiagnosis,Tumor Hospital of Jilin Province，Changchun 130012,China

Received:2006-08-18 Revised:1900-01-01 Online:2007-05-28 Published:2007-05-28
Contact: ZHENG Yong-chen

摘要/Abstract

摘要： 目的：探讨临床白色念珠菌感染者不同标本中分离的白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶活性与其毒力的关系。方法：分别采用牛奶平板和卵黄培养基法检测白色念珠菌感染者不同标本（痰、血液、鹅口疮、伤口、阴道分泌物）中分离的190株白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶的活力，分别将产酶株的菌丝相和孢子相菌悬液（5×10⁶ CFU•mL^-1）注射小鼠尾静脉，1个月内观察小鼠死亡率及平均存活时间，以平均存活时间评价菌株毒力。结果：190株白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶阳性检出率分别为83.68%和85.26%。动物实验结果表明，菌丝相白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶的活力均显著高于孢子相(P<0.01，P＜0.05)；注射菌丝相白色念珠菌的小鼠死亡率高于注射孢子相的小鼠(P<0.05)，且平均存活期短于注射孢子相的小鼠(P<0.01)。结论：分泌型酸性蛋白酶和细胞外磷脂酶是白色念珠菌重要毒力因子，酶活力可直接地反映其毒力和致病性。

关键词: 白色, 分泌型酸性蛋白酶, 细胞外磷脂酶, 毒力

Abstract: To study the relationship between activity and virulence of secretory acid proteinase and extracellular phosphlipase of different Candida albicans isolated from various specimens .Methods 190 strains of Candida albicans were isolated from various specimens (sputum,blood,thrush,wound,secretion of vaginitis) of patients with Candida albicans infection in hospital. Milk-plate medium and egg-yolk medium were used respectively to test the activities of secretory acid proteinase and extracellular phosphlipase of Candida albicans.The suspension of two forms of Candida albicans （5×10⁶CFU•mL^-1） were injected to vein of tail in mice respectively in virulence test.The mortality and mean survival time of mice were observed in 1 month.The virulence was appreciated with the mean survival time of mice. Results All of 190 strains，the positive detectable rates of secretory acid proteinase and extracellular phosphlipase were 83.68% and 85.26%，respectively.Animal experimental results showed the activities of secretory acid proteinase and extracellular phospolipase in hyphal forms were significantly higher than that in spore form(P<0.01,P<0.05).The mortality of mice injected with hyphal forms was higher than that of mice injected with spore forms (P<0.05)and the mean survival time of mice injected with hyphal forms was shorter than that of mice injected with spore forms(P<0.01). Conclusion Secretory acid proteinase and extracellular phosphlipase are important virulence factors of Candida albicans.The activities of secretory acid proteinase and extracellular phosphlipase can be used as direct parameters for virulence of Candida albicans.

Key words: secretory acid proteinase, extracellular phospholipase, virulence

中图分类号:

R379.4

曹岩，张巨，沙春蕊，郑永晨，刘永红. 白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶活性与其毒力的关系[J]. J4, 2007, 33(3): 483-487.

CAO Yan， ZHANG Ju， SHA Chun-rui， ZHENG Yong-chen， LIU Yong-hong. Relationship between activity and virulence of secretory acid proteinaseand extracellular phosphlipase of Candida albicans[J]. J4, 2007, 33(3): 483-487.

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