生长激素对大鼠急性胃黏膜病变的保护作用

doi:吉林省科技厅资助课题(20050702)

生长激素对大鼠急性胃黏膜病变的保护作用

宋晓斌¹，张学春¹，张平¹，马震²，更伟²

1.吉林大学第一医院普通外科，吉林长春 130021； 2.吉林省人民医院急诊外科，吉林春 130021

收稿日期:2005-12-13 修回日期:1900-01-01 出版日期:2007-05-28 发布日期:2007-05-28
通讯作者: 张学春

Theraputic effect of growth hormone on acute gastric mucosal lesion in rats

SONG Xiao-bin¹,ZHANG Xue-chun¹，ZHANG Ping¹,MA Zhen²,ZHANG Geng-wei²

1.Department of General Surgery,First Hospital,Jilin University,Changchun 130021,china; 2.Department of Emergency Surgery,People’s Hospital of Jilin Province,Changchun 130021,china

Received:2005-12-13 Revised:1900-01-01 Online:2007-05-28 Published:2007-05-28
Contact: ZHANG Xue-chun

摘要/Abstract

摘要： 目的：探讨生长激素对急性胃黏膜病变（AGML，又称应激性溃疡）的保护作用。方法：120只Wistar大鼠随机分为空白对照组(n=8)、单纯AGML模型组(n=32)、AGML模型+生长激素(rhGH)组(n=40)、AGML模型+西咪替丁阳性对照组(n=40)。采用水浸束缚法建立大鼠AGML模型，在此病理模型基础上应用重组人生长激素（rhGH，1U•kg^-1•d^-1）治疗大鼠AGML，光镜和电镜下观察应激0、4、8及12 d后各组大鼠胃黏膜的形态学改变，同时对比胃泌酸量、pH值、胃黏膜厚度及溃疡指数等指标。结果：与空白对照组和AGML模型+西咪替丁组比较，光镜下，AGML模型+rhGH组大鼠胃黏膜的组织病理学改变明显减轻，仅部分上皮肿胀，偶见红细胞渗出，中性粒细胞浸润明显减少，黏膜下层结构无明显改变；电镜下，胃黏膜壁细胞、主细胞状态良好，仅少量细胞出现变性改变; AGML模型+rhGH组胃黏膜萎缩程度及溃疡指数亦低于上述两组（P<0.05），且随用药时间的延长黏膜病变有逐渐减轻的趋势，这种差别在应激的第8天时已趋于明显（P<0.05）。结论：生长激素可以刺激胃肠道黏膜上皮再生、修复，促进胃黏膜屏障结构和功能恢复，从而对AGML起到治疗作用。

关键词: 生长激素(人), 西咪替丁

Abstract: To discuss the effect of growth hormone (GH) on acute gastric mucosal lesion (AGML) in rats.Methods 120 Wistar rats were divided into four groups randomly: blank control group(n=8),simple model group(n=32),model+rhGH group(n=40) and model+cimetidine group(n=40).Animal models of AGML were set up through soak and tie of rats.GH was injected to the rats in experimental groups,while cimetidine was used in the positive control group.Morphologic changes of rat gastric mucosa in each group were observed and compared on different days(0,4,8,12 d) after stress through gross looking,light microscope,and electron microscope.Ulcer index(UI),secretion amount of gastric acid,pH value and gastric mucosal were compared.Results Compared with blank control and model+cimetidine groups,the histomorphologic changes of gastric mucosa were obviously alleviated in model+rhGH group: only part of epithelial cells swelled,red blood cells were seldom seen,neutrophils infiltration reduced obviously,no remarkable changes were observed in the submucosal tissue.Mucosal cells were in good state and degeneration were seen in only a few cells under the electronic microscope.In addition,UI and the degree of gastric mucosal atrophy in model+rhGH group were lower than that in control group significantly(P<0.05),and the difference became more and more obvious with the prolongation of duration of administration of GH. Conclusion GH can strengthen the mucosal barrier by promoting the growth and reparation of gastric mucosa,and promote restoration of gastric mucosa in both structure and function,thereby play curative effect on AGML.

Key words: somatropin, cimetidine

中图分类号:

R573.1

宋晓斌，张学春，张平，马震，更伟. 生长激素对大鼠急性胃黏膜病变的保护作用[J]. J4, 2007, 33(3): 503-507.

SONG Xiao-bin,ZHANG Xue-chun，ZHANG Ping,MA Zhen,ZHANG Geng-wei. Theraputic effect of growth hormone on acute gastric mucosal lesion in rats[J]. J4, 2007, 33(3): 503-507.

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