DNA修复酶MGMT及Ki-67在星形细胞瘤中的表达及临床意义

doi:国家自然科学基金资助课题（30370669）

DNA修复酶MGMT及Ki-67在星形细胞瘤中的表达及临床意义

王丽萍¹,孙逊¹,邹亚彬¹,张俊阁¹,李波²,曹阳^*

1.吉林大学第一医院病理科,吉林长春 130021;2.吉林大学公共卫生学院流行病与卫生统计学教研室,吉林长春 130021

收稿日期:2006-09-11 修回日期:1900-01-01 出版日期:2007-05-28 发布日期:2007-05-28
通讯作者: 李波

Expressions of DNA repair enzyme MGMT and Ki-67 in human astrocytoma 　and clinical significances

WANG Li-ping¹,SUN Xun¹,ZOU Ya-bin¹,ZHANG Jun-ge¹,LI bo²，CAO Yang^*

1.Department of Pathology,First Hospital,Jilin University,　Chang chun 130021,China;2.Department of Epidemiology and Health Statistics,School of Public Health,Jilin University,Changchun 130021,China

Received:2006-09-11 Revised:1900-01-01 Online:2007-05-28 Published:2007-05-28
Contact: LI bo

摘要/Abstract

摘要： 目的：观察脑星形细胞瘤中DNA修复酶—6氧-甲基鸟嘌呤DNA甲基转移酶（MGMT）及细胞增殖核抗原（Ki-67）蛋白表达及临床意义。方法：69例样本均来源于神经外科手术切除且临床资料完整、术后病理诊断为星形细胞瘤病例，其中Ⅰ～Ⅱ级34例，Ⅲ级21例，Ⅳ级14例。应用免疫组织化学方法(ABC法)检测样本中MGMT及Ki-67蛋白的表达状况，并进行秩和相关等级检验。结果：星形细胞瘤中MGMT蛋白表达阳性率为60.9％，其中， Ⅰ～Ⅱ级、Ⅲ级和Ⅳ级分别为88.2％、23.5％和50.0％。不同分级之间MGMT蛋白表达比较差异均有显著性（P＝0.003 1），MGMT阳性表达率与肿瘤分级呈负相关（r＝－0.351 1，P＝0.003 1）；星形细胞瘤中Ki-67阳性率为62.3％，随肿瘤恶性程度的增加蛋白表达明显增强（P=0.000 0），该表达与肿瘤分级呈正相关（r＝0.690 4，P=0.000 0）；并且随Ki-67表达强度的增加，MGMT的表达强度明显下降（P＝0.015 1），Ki-67与MGMT蛋白表达呈负相关（r＝－0.291 5，P＝0.015 1）。结论：星形细胞瘤中MGMT及 Ki-67表达与肿瘤恶性程度密切相〖JP3〗关，提示MGMT和Ki-67可以作为评价星形细胞瘤预后的生物学指标。〖JP〗

关键词: 6氧-甲基鸟嘌呤DNA甲基转移酶, 细胞增殖核抗原

Abstract: Abstract:Objective To explore the expressions of DNA repair enzyme 6-methyiguanine-DNA methyltransferase（MGMT） and cell multiplication nuclear antigen ( Ki-67) in human astrocytomas and clinical significances．Methods Sixty  nine specimens of human astrocytomas (grade Ⅰ-Ⅱ: 34 cases, grade Ⅲ:21 cases,grade Ⅳ:14 cases) were selected.The expressions of MGMT and Ki-67 were observed with immunohistochemical technique,and the relation between MGMT and Ki-67 was evaluated.Results The positive rate of MGMT in astrocytoma was 60.9%, the positive rates of grade Ⅰ-Ⅱ,grade Ⅲ and grade Ⅳ were 88.2%，23.5% and 50.0%,respectively.There were significant differences between various grades of tumor （P=0.003 1），and the expression of MGMT had negative correlation with tumor grades （r=－0.351 1）.The positive rate of Ki-67 was 62.3%,and obviously increased with the grades of tumor （P=0.000 0)，and the expression of ki-67 had positive correlation with tumor grades（r=0.690 4, P=0.000 0）; the expression of MGMT decreased with the increase of expression of Ki-67（P=0.051）,the expression of MGMT had negative correlation with Ki-67 expression （r＝－0.291 5,P＝0.015 1）. Conclusion The expressions of MGMT and Ki-67 have close correlation with the degrees of malignancy in human astrocytomas.MGMT and Ki-67 may be used as biological parameters to estimate the prognosis of astrocytoma.

Key words: 6-methyiguanine-DNA methyltransferase , cell multiplication nuclear antigen

中图分类号:

R739.4

王丽萍,孙逊,邹亚彬,张俊阁,李波,曹阳. DNA修复酶MGMT及Ki-67在星形细胞瘤中的表达及临床意义[J]. J4, 2007, 33(3): 549-551.

WANG Li-ping,SUN Xun,ZOU Ya-bin,ZHANG Jun-ge,LI bo，CAO Yang. Expressions of DNA repair enzyme MGMT and Ki-67 in human astrocytoma 　and clinical significances[J]. J4, 2007, 33(3): 549-551.