嘌呤核苷酸减弱吗啡抑制的PC12细胞嘌呤核苷酸合成代谢

doi:吉林省科技厅资助课题（200505229）

嘌呤核苷酸减弱吗啡抑制的PC12细胞嘌呤核苷酸合成代谢

孙婷，何海涛，阚慕洁，张连芝，洪敏

（吉林大学基础医学院生物化学教研室，吉林长春 130021 ）

收稿日期:2006-12-08 修回日期:1900-01-01 出版日期:2007-11-28 发布日期:2007-11-28
通讯作者: 洪敏

Effects of purine nucleotides on purine nucleotide anabolism down-regulation by morphine in PC12 cells

SUN Ting,HE Hai-tao,KAN Mu-jie,ZHANG Lian-zhi,HONG Min

(Department of Biochemistry,School of Basic Medical Sciences,Jilin University,Changchun 130021,China)

Received:2006-12-08 Revised:1900-01-01 Online:2007-11-28 Published:2007-11-28
Contact: HONG Min

摘要/Abstract

摘要： 目的：探讨外源性嘌呤核苷酸对吗啡致神经细胞核苷酸合成代谢降低的影响。方法: 大鼠肾上腺嗜铬细胞瘤(PC12细胞)分为对照组、吗啡组、单嘌呤核苷酸（AMP+GMP）组、三嘌呤核苷酸（ATP+GTP）组、吗啡+AMP+GMP组及吗啡+ATP+GTP组。应用RT-PCR法检测各实验组腺苷激酶（AK）和次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）基因转录产物含量的变化。结果:吗啡组AK mRNA及HGPRT mRNA表达量（分别为1.330±0.108和1.407±0.141）与对照组（1.874±0.161和1.923±0.155）比较明显降低(P<0.01,P<0.05)。吗啡+AMP+GMP组AK mRNA表达量（1.626±0.171）与吗啡组和对照组比较差异无显著性（P>0.05），HGPRT mRNA表达量（1.796±0.168）高于吗啡组(P<0.05)。吗啡+ATP+GTP组AK mRNA表达量（1.107±0.164）低于对照组(P<0.01),HGPRT mRNA表达量（1.563±0.209）与吗啡组及对照组比较差异无显著性（P>0.05）。结论：外源性补充嘌呤核苷酸能改善吗啡所致的PC12细胞嘌呤核苷酸合成代谢关键酶基因表达降低。

关键词: 吗啡, PC12细胞, 腺苷激酶, 次黄嘌呤磷酸核糖转移酶

Abstract: To investigate the effect of exogenous purine nucleotides on neuron nucleotide anablism depression. Methods Rat pheochromocytoma (PC12 cells) were divided into control,morphine,mono-purine nucleotide(AMP+GMP),tri-purine nucleotide(ATP+GTP),morphine+AMP+GMP and morphine+ATP+GTP groups.RT-PCR was used to examine the gene transcripts concentrations of adenosine kinase (AK) and hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in each group. ResultsThe concentrations of AK and HGPRT mRNA(1.330±0.108 and 1.407±0.141 respectively) were decreased in morphine group (P<0.01,P<0.05) compared with control group(1.874±0.161 and 1.923±0.155 respectively).The expression level of AK mRNA in the group of morphine+AMP+GMP (1.626±0.171) had no difference compared with control and morphine group(P>0.05),and HGPRT mRNA expression level (1.796±0.168) was higher than that in morphine group(P<0.05).The expression level of AK mRNA in the group of morphine+ATP+GTP (1.107±0.164) was lower than that in control group(P<0.01),and HGPRT mRNA expression level (1.563±0.209) had no difference compared with control and morphine group(P>0.05). ConclusionThe exogenous supply of purine nucleotides can ameliorate the down-regulation of morphine on purine nucleotide anabolism key enzyme gene expression in PC12 cells.

Key words: morphine, PC12 cells, adenosine kinase, hypoxanthine phosphoribosyltransferase

中图分类号:

R730.264

孙婷，何海涛，阚慕洁，张连芝，洪敏. 嘌呤核苷酸减弱吗啡抑制的PC12细胞嘌呤核苷酸合成代谢[J]. J4, 2007, 33(6): 971-973.

SUN Ting,HE Hai-tao,KAN Mu-jie,ZHANG Lian-zhi,HONG Min. Effects of purine nucleotides on purine nucleotide anabolism down-regulation by morphine in PC12 cells[J]. J4, 2007, 33(6): 971-973.

[1]	徐姗姗, 朴美花, 王艳姝, 刘楠, 裴爱月, 冯春生. 异氟醚对Aβ25-35诱导大鼠PC12细胞氧化应激损伤的影响及海藻糖的保护作用[J]. 吉林大学学报(医学版), 2015, 41(02): 207-212.
[2]	邹泳国，周春燕，朴美花，刘楠，岳云，冯春生. 异氟醚对β淀粉样蛋白诱导的大鼠PC12细胞凋亡和内质网应激的影响及其机制[J]. 吉林大学学报(医学版), 2014, 40(02): 224-228.
[3]	王伟，朴美花，王艳姝，刘楠，岳云，冯春生. 异氟醚对Aβ25-35诱导大鼠PC12细胞自噬和凋亡的影响[J]. 吉林大学学报(医学版), 2014, 40(01): 49-54.
[4]	姜恩平, 李红, 杨世杰. 蒺藜皂苷对氧化应激PC12细胞内NF-κB水平的影响及其机制[J]. J4, 2010, 36(6): 1035-1038.
[5]	姜恩平, 吴金义, 陈建光. 北五味子总木脂素对H₂O₂诱导PC12细胞凋亡的影响[J]. J4, 2009, 35(3): 466-469.
[6]	王少华，孙吉凤，刘剑凯，张连芝，邓志会. 吗啡对C6神经胶质瘤细胞Glu和Gln含量及其代谢关键酶转录活性的影响[J]. J4, 2008, 34(5): 786-789.
[7]	姜恩平，李红，纪影实，杨世杰. 蒺藜总皂苷对H₂O₂诱导PC12细胞凋亡的保护作用[J]. J4, 2008, 34(2): 183-186.
[8]	王秀力，李忱，刘佳梅，陈东. 维甲酸对诱导PC12细胞向神经元样细胞分化的影响[J]. J4, 2008, 34(2): 187-190.
[9]	张海娜，胡国华，陈秋惠，孙冬，刘彩霞，孙亚娟. 帕金森病细胞模型的建立及鱼藤酮对多巴胺能神经元的毒性作用[J]. J4, 2007, 33(5): 811-814.
[10]	王秀力，陈东，刘佳梅. NGF诱导PC12细胞向神经元的分化[J]. J4, 2007, 33(5): 827-830.
[11]	韩威, 宫萍, 常明, 张瑜, 张颖, 王秋艳, 胡轶虹,胡林森. 鱼藤酮对PC12细胞毒性作用的机制[J]. J4, 2007, 33(1): 98-101.
[12]	袁海英，刘英鹰，刘剑凯，洪敏. 吗啡对PC12细胞嘌呤核苷酸代谢相关酶基因表达的影响[J]. J4, 2006, 32(6): 1037-1040.
[13]	张磊, 常明, 徐卉, 侯澍, 李红杰, 朴燕洁, 胡林森. PC12细胞蛋白质组学研究技术及二维电泳图谱的建立[J]. J4, 2006, 32(6): 1112-1115.
[14]	刘剑凯，洪敏，阚慕洁，张纪周，张莲芝，袁海英. 嘌呤对慢性吗啡处理大鼠热痛阈与急性戒断的影响[J]. J4, 2006, 32(4): 615-617.
[15]	房绍宽，吴江，周春奎. 新型抗精神失常药万拉法辛和奥氮平对无血清条件下PC12细胞的保护作用[J]. J4, 2006, 32(3): 480-482.