含鼠白细胞介素12基因腺病毒载体对小鼠支气管哮喘模型肺组织GATA-3表达的影响

doi:吉林省科技厅基金资助课题（19980321-2）

含鼠白细胞介素12基因腺病毒载体对小鼠支气管哮喘模型肺组织GATA-3表达的影响

车广华¹, 高航², 王建伟²,张海英²，鲁继荣³ 

（1. 吉林大学第二医院儿科,吉林长春 130041； 2. 吉林大学基础医学院病理生物学教育部重点实验室,吉林长春 130021； 3.吉林大学第一医院儿科，吉林长春 130021）

收稿日期:2007-04-25 修回日期:1900-01-01 出版日期:2007-11-28 发布日期:2007-11-28
通讯作者: 王建伟

Effects of adenovirus vector carrying murine IL-12 gene on expression of GATA-3 in lung tissue of mouse model with asthma

CHE Guang-hua¹,GAO Hang²,WANG Jian-wei²,ZHANG Hai-ying²，LU Ji-rong³

(1.Department of Pediatrics,Second Hospital,Jilin University,Changchun 130041,China;2. Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3. Department of Pediatrics,First Hospital,Jilin University,Changchun 130021,China)

Received:2007-04-25 Revised:1900-01-01 Online:2007-11-28 Published:2007-11-28
Contact: WANG Jian-wei

摘要/Abstract

摘要： 目的：探讨含鼠白细胞介素12（IL-12）基因的腺病毒载体（AdCMVIL-12）对小鼠支气管哮喘模型GATA-3表达的影响。方法：BALB/c小鼠30只,随机分为正常对照组（A组）、哮喘模型组（B组）和AdCMVIL-12实验组（C组），B和C组建立哮喘模型，并于第20天分别鼻内吸入50 μL生理盐水和5×10⁸ PFU AdCMVIL-12。第28天收集各组小鼠的支气管肺泡灌洗液（BALF），比较细胞总数及细胞分类的变化，HE染色观察小鼠支气管及肺组织的病理变化，免疫组化及RT-PCR方法检测各实验组肺组织中GATA-3的表达情况。结果：哮喘模型组与对照组及AdCMVIL-12实验组比较，小鼠肺组织有大量炎症细胞浸润，以嗜酸性粒细胞为主。免疫组织化学及RT-PCR结果显示，哮喘模型组小鼠肺组织GATA-3表达（0.48 ±0.06）明显高于实验组（0.16±0.07）及对照组（0.18±0.04 ）(P<0.01)。结论：AdCMVIL-12对小鼠支气管哮喘的气道及肺组织炎症有明显的抑制作用，其机制可能与AdCMVIL-12阻断GATA-3的表达,调节Th₁/Th₂细胞因子的平衡有关。

关键词: GATA-3, AdCMVIL-12, 哮喘模型, 基因疗法

Abstract: To investigate the effects of adenovirus vector carrying murine interleukin-12 gene (AdCMVIL-12) on the expression of GATA-3 in mouse model with asthma. MethodsThirty BALB/c mice were randomly divided into 3 groups: control group（group A），asthma model group (group B) and AdCMVIL-12 experimental group (group C).Asthma models were established in group B and C,saline (50 μL) and 5×10⁸ PFU AdCMVIL-12 were given by intranasal administration at the 20th days.BALF were recovered at the 28th day for cellular classification.Pathological changes of bronchial and lung tissue were observed by HE staining in each groups.The expression of GATA-3 in each group was detected by immunohistochemistry and RT-PCR. Results Compared　 with group A and C,a lot of inflammatory cells were found in group B and the percent of eosinophils was the most,the expression of GATA-3 in lung tissue of group B(0.48 ±0.06) was higher than those in group C(0.16±0.07) and group A(0.18±0.04) (P<0.01). Conclusion AdCMVIL-12 can significantly inhibit airway and lung tissue inflammation in asthma model,its mechanism may be related to blocking the expression of GATA-3 in asthma model and regulating the balance of Th₁/Th₂ cytokines.

Key words: GATA-3, AdCMVIL-12, asthma model, gene therapy

中图分类号:

R332

车广华, 高航, 王建伟,张海英，鲁继荣 . 含鼠白细胞介素12基因腺病毒载体对小鼠支气管哮喘模型肺组织GATA-3表达的影响[J]. J4, 2007, 33(6): 974-977.

CHE Guang-hua,GAO Hang,WANG Jian-wei,ZHANG Hai-ying，LU Ji-rong. Effects of adenovirus vector carrying murine IL-12 gene on expression of GATA-3 in lung tissue of mouse model with asthma[J]. J4, 2007, 33(6): 974-977.

[1]	杨志广, 刘林林, 邵国光. 短双歧杆菌携带内皮抑素和干扰素γ的特性及其抗小鼠肺癌的作用[J]. J4, 2011, 37(4): 596-602.
[2]	王健君，李艳茹，李晓敏，张海英，王琳. 水流动力学注射介导的IL-12基因对小鼠恶性腹水的治疗作用[J]. J4, 2008, 34(2): 179-182.
[3]	汪杨，牛俊奇，王峰. RNA干涉对乙型肝炎病毒复制和表达的影响[J]. J4, 2007, 33(4): 616-620.
[4]	杨英,付士波, 刘树铮. IL-12联合B7-1基因放射治疗对小鼠B16移植肿瘤生长的影响[J]. J4, 2006, 32(6): 947-949.
[5]	高航，王建伟，车广华，张海英，陈远耀. 重组鼠白细胞介素12与粒细胞-巨噬细胞集落刺激因子基因联合治疗小鼠B16黑色素瘤[J]. J4, 2006, 32(6): 996-999.
[6]	王建伟，高航，高嵩，丛建军，陈远耀. 含鼠GM-CSF基因的腺病毒载体对荷B16黑色素瘤小鼠免疫功能的影响[J]. J4, 2005, 31(4): 533-535.
[7]	田梅，朴春姬，李修义，杨巍. 电离辐射诱导pEgr-hPTEN 表达增强其体外抗肿瘤作用[J]. J4, 2005, 31(3): 330-333.
[8]	张绍昆，高萍，张琪，刘一，徐莘香. 人胰岛素样生长因子-1及绿色荧光蛋白双表达载体的构建[J]. J4, 2005, 31(1): 152-154.
[9]	宋志宇，张家颖，赵忠文. 转染血管内皮生长因子基因治疗勃起功能障碍[J]. J4, 2004, 30(1): 157-159.
[10]	王美霞，牛俊奇，王峰. 以U₁小核核糖核酸为靶向基因构建HCV特异性嵌合体核酶[J]. J4, 2004, 30(1): 23-26.