地塞米松对体外培养人牙周膜成纤维细胞增殖的影响

doi:吉林省科技厅基金资助课题(20020616)

地塞米松对体外培养人牙周膜成纤维细胞增殖的影响

罗云纲¹，刘晓秋²，朱孝民³，阎晓冬⁴

1.吉林大学第二医院口腔科，吉林长春 130041；2. 吉林大学口腔医院修复科，吉林长春 130041；3. 吉林大学第二医院医务科，吉林长春 130041；4.吉林省长春市中心医院口腔科，吉林长春 130051

收稿日期:2007-10-26 修回日期:1900-01-01 出版日期:2008-05-28 发布日期:2008-05-28
通讯作者: 罗云纲

Effect of dexamethasone on proliferation of human periodontal ligament fibroblasts cultivated in vitro

LUO Yun-gang¹,LIU Xiao-qiu²,ZHU Xiao-min³,YAN Xiao-dong¹

1.Department of Stomatology,Second Hospital,Jilin University,Changchun 130041,China; 2.Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130041,China；3. Department of Medical Affairs，Second Hospital,Jilin University,Changchun 130041,China；4. Department of Stomatology,Changchun Central Hospital,Changchun 130051,China

Received:2007-10-26 Revised:1900-01-01 Online:2008-05-28 Published:2008-05-28
Contact: LUO Yun-gang

摘要/Abstract

摘要： 目的：研究地塞米松（DEX）对体外培养人牙周膜成纤维（PDLF）细胞增殖的影响，寻找体外培养人PDLF(hPDLF)细胞的优化条件,为牙周组织再生的研究工作奠定基础。方法：体外分离培养的hPDLF细胞随机分为5组,分别用5种含10%胎牛血清的DMEM细胞培养基培养5～8代:①空白对照组（无DEX）；②含5 mg•L^-1DEX；③含10 mg•L^-1 DEX；④含20 mg•L^-1 DEX；⑤含50 mg•L^-1 DEX。以MTT法测定DEX对hPDLF细胞增殖率的影响；采用倒置相差显微镜观察hPDLF细胞在添加不同浓度DEX的胎牛血清培养基中培养后的细胞形态学改变。结果：将hPDLF细胞接种于24孔板后的第3天，细胞出现汇合现象。细胞在孔底呈鳞片状。采用DEX条件DMEM细胞培养液培养，可见细胞逐渐变成多层，细胞形态变小，突起变钝。MTT法检测结果表明含不同浓度DEX的DMEM培养基中培养的hPDLF细胞数均高于对照组（P<0.05）；不同浓度的DEX对hPDLF细胞增殖均有影响(P<0.05），且随着浓度增加，hPDLF细胞增殖能力逐渐增高。结论：DEX能使体外培养的hPDLF细胞表现很强的增殖活性。

关键词: 体外培养, 胎牛血清, 地塞米松

Abstract: Abstract:Objective To investigate the effect of dexamethasone on proliferation of human periodontal ligament fibroblasts(hPDLF) cultivated in vitro,and search for optimal culture condition for hPDLF in vitro， and provide basis for further study on regeneration of periodontinum.Methods The hPDLF cultivated in vitro were divided into 5 groups and cultivated in 5 different culture media separately which all included 10% fetal bovine serium: ①control group(no DEX）；②5 mg•L^-1DEX；③10 mg•L^-1 DEX；④20 mg•L^-1 DEX；⑤50 mg•L^-1 DEX. The proliferation of hPDLF was assyed by MTT method.The phase contrast microscope was used to observe the morphological changes of the cells.Results The PDLF appeared aggregation and cells on bottom of culture bottle showed squamae-shape after inoculated on plate with 24 hole for 3 d.There were more layers of smaller hPDLF with blunt prominency came forth after cultivated in DEX culture media.MTT method showed that DEX promoted the proliferation of hPDLF obviously compared with control group(P<0.05).The different concentrations of DEX had different effects on proliferation of hPDLF(P<0.05),the proliferation ability was enhanced with the increasing of DEX doses.Conclusion DEX could improve the proliferation ability of hPDLF cultivated in vitro.

Key words: culture in vitro, fetal bovine serium, dexamethasone

中图分类号:

R977.1

罗云纲，刘晓秋，朱孝民，阎晓冬. 地塞米松对体外培养人牙周膜成纤维细胞增殖的影响[J]. J4, 2008, 34(3): 462-465.

LUO Yun-gang,LIU Xiao-qiu,ZHU Xiao-min,YAN Xiao-dong. Effect of dexamethasone on proliferation of human periodontal ligament fibroblasts cultivated in vitro[J]. J4, 2008, 34(3): 462-465.

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