牙髓炎患者牙髓组织中单核细胞趋化蛋白1的表达及意义

doi:吉林省科技厅基金资助课题(953460-2)

牙髓炎患者牙髓组织中单核细胞趋化蛋白1的表达及意义

张志民¹,高心¹,王成坤¹,张筠英²

（1.吉林大学口腔医院牙体牙髓病科，吉林长春 130041；2.吉林省长春市口腔医院牙体牙髓病科，吉林长春 130041）

收稿日期:2008-01-17 修回日期:1900-01-01 出版日期:2008-11-28 发布日期:2008-11-28
通讯作者: 高心

Expression of monocyte chemoattractant protein-1 in pulp tissue in patients with pulpitis and its significance

ZHANG Zhi-min¹，GAO Xin¹,WANG Cheng-kun¹,ZHANG Yun-ying²

（1.Deparment of Endodontics and Operative Dentistry,Stomatology Hospital,Jilin University,Changchun 130041,China;2. Deparment of Endodontics and Operative Dentistry,Stomatological Hospital of Changchun,Changchun 130041,China）

Received:2008-01-17 Revised:1900-01-01 Online:2008-11-28 Published:2008-11-28
Contact: GAO Xin

摘要/Abstract

摘要： 目的:探讨牙髓炎患者牙髓组织中单核细胞趋化蛋白1 （MCP-1）的表达，揭示MCP-1在牙髓炎性疾病发生、发展中的作用机理。方法：应用免疫组织化学方法检测MCP-1在正常人健康牙髓（正畸或烤瓷牙需要拔髓者）、急性及慢性牙髓炎患者牙髓组织(各30例)中的分布。结果:在健康牙髓组织中未发现MCP-1表达阳性细胞。在急性牙髓炎症组织中，可见大量的中性粒细胞游出血管并向炎症中心趋化，MCP-1阳性细胞主要分布于中性粒细胞、单核细胞、巨噬细胞，阳性细胞平均灰度值119.73±17.12。在慢性牙髓炎症组织中，可见血管内皮细胞、巨噬细胞、中性粒细胞、成纤维细胞、新生的毛细血管上MCP-1有不同程度的阳性表达，阳性细胞平均灰度值121.26±2.04，与急性牙髓炎症组织阳性细胞平均灰度值比较差异无显著性(P>0.05)。结论：MCP-1在牙髓炎牙髓组织中有表达，在牙髓炎发生、发展过程中可能促进炎症组织的愈合。

关键词: 牙髓炎

Abstract: To investigate the expression of monocyte chemoattractant protein-1(MCP-1) in pulp tissue in patitents with pulpitis,and elucidate its role and mechanism in occurrence and development of pulpitis.Methods Using immunohistochemical staining method,the expression and distribution of MCP-1 in 30 cases of normal pulps,30 acute and 30 chronic pulpitis tissues were detected.Results Different extent of positive MCP-1 expression was detected in acute and chronic pulpitis tissues,but no expression was detected in normal pulp tissue.The yellow particles in cytoplasm were the positive expression,which located in leukocytes,monocytes and macrophages in acute pulpitis tissue.In chronic pulpitis tissue the positive cells were vascular endothelial cells,macrophages,leukocytes,and so on.The extent of positive MCP-1 in acute pulpitis tissue was 119.73±17.12 and the extent of positive MCP-1 in chromic pulpitis tissue was 121.26±2.04,the difference was not significant(P>0.05).Conclusion MCP-1 expresses in pulp tissue in patients with pulpitis.MCP-1 could conduce the pulpitis tissue to heal in the occurrence and development of pulpitis.

Key words: pulpitis

中图分类号:

R781.1

张志民,高心,王成坤,张筠英. 牙髓炎患者牙髓组织中单核细胞趋化蛋白1的表达及意义[J]. J4, 2008, 34(6): 1035-1037.

ZHANG Zhi-min，GAO Xin,WANG Cheng-kun,ZHANG Yun-ying. Expression of monocyte chemoattractant protein-1 in pulp tissue in patients with pulpitis and its significance[J]. J4, 2008, 34(6): 1035-1037.