关键词: 重组人粒细胞-巨噬细胞集落刺激因子, 创伤愈合, 牙龈创伤

Abstract:

Abstract:Objective To investigate the effects of recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) on the gingival wound healing，and to lay a foundation for its application in the field of oral cavity.Methods 16 healthy rabbits were randomly divided into experiment and control groups(n=8).2　mm upper and lower incisors’ labial gingivae were removed by scalpel.After operation,rhGM-CSF gel was applied to the surface of the wound gum in experiment group,while saline instead of drugs to the control group.2 rabbits were executed at 3,7,11,and 15 d after operation.HE staining was performed to observe the epithelium and epithelial connective tissue structure,distribution,and the changes of various cells of the rats;the  number of positive epithelial cells and fibroblasts were observed by PCNA dyeing.Results On the 3rd day, the  inflammatory cell infiltration was found in two groups,but a greater number of the cells were observed in experiment group; neovascularization was seen in both groups on the 7th day,and the fibroblasts and collagen fibers were observed too,but the extent of neovascularization in experiment group was more significant.On the 11th day,the fibroblasts proliferation was seen in two groups,but the extent in experiment group was more widely; on the 15th day,the trauma of gingivae of the rabbits in two  groups returned to normal,there was no significant difference between two groups.Over time,the number of epithelial cells in proliferation was gradually increased,reached the peak on the 15th day.The number of fibroblasts began to increase from the 3rd day and reached the peak on the 11th day,and significantly reduced to the lowest value  on the 15th day.The number of proliferative epithelial cells in experiment group was significantly higher (P<0.01)on the 3rd day and the 11th day than those in control group(P<0.01);the number of proliferative  fibroblasts was significantly higher (P<0.05) on the 11th day and the 15th day than those in control group(P<0.05),but there were no significantly statistical differences between two groups at other time pionts(P>0.05).Conclusion  RhGM-CSF can promote the infiltration of inflammatory cells,angiogenesis, proliferation of epithelial cells and fibroblasts in gingivae.RhGM-CSF is beneficial with wound healing on gum tissue.

Key words: recombinant human granulocyte-macrophage colony stimulating factor;wound healing, gum trauma