可溶性Tim-3对原因不明性复发性流产患者Th1/Th2分化的免疫调节作用及其机制

doi:10.13481/j.1671-587x.20160622

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (06): 1163-1167.doi: 10.13481/j.1671-587x.20160622

可溶性Tim-3对原因不明性复发性流产患者Th1/Th2分化的免疫调节作用及其机制

和秀魁^1,2, 陶莉莉¹, 袁晓兰², 齐一鸣², 罗喜平², 潘碧琦³

1. 广州中医药大学第一临床医学院妇科, 广东广州 510405;
2. 广东省妇幼保健院广州医学院附属广东省妇儿医院妇产科, 广东广州 511442;
3. 广东省妇幼保健院广州医学院附属广东省妇儿医院中医科, 广东广州 511442

收稿日期:2015-03-04 出版日期:2016-11-28 发布日期:2016-12-02
通讯作者: 陶莉莉,教授,主任医师,博士研究生导师(Tel:020-36591360,E-mail:2762204682@qq.com) E-mail:2762204682@qq.com
作者简介:和秀魁(1973-),女,广东省信宜市人,主任医师,医学博士,主要从事妇科内分泌、普通妇科和中西医结合妇科方面的研究。
基金资助:
广东省中医药局科研项目资助课题（20152022）

Immunoregulatory effectof soluble Tim-3 on Th1/Th2 differentiation in patients with unexplained recurrent spontaneous abortion and its mechanism

HE Xiukui^1,2, TAO Lili¹, YUAN Xiaolan², QI Yiming², LUO Xiping², PAN Biqi³

1. Department of Gynecology, First Clinical Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China;
2. Department of Gynecology, Guangdong Women and Child Hospital, Guangdong Provincial Maternity and Child Care Center, Guangzhou Medical College, Guangzhou 511442, China;
3. Department of Traditional Chinese Medicine, Guangdong Women and Child Hospital, Guangdong Provincial Maternity and Child Care Center, Guangzhou Medical College, Guangzhou 511442, China

Received:2015-03-04 Online:2016-11-28 Published:2016-12-02

摘要/Abstract

摘要：

目的：观察可溶性T细胞免疫球蛋白黏蛋白分子3（sTim-3）在原因不明性复发性流产（URSA）患者血清中表达水平的变化及其对Th1/Th2细胞分化的调节，探讨sTim-3在URSA病理过程中的免疫机制。方法：选择52例URSA患者作为URSA组，选取同期正常妊娠孕妇20人作为正常妊娠组，育龄非孕者20人为健康对照组。采用ELISA法检测3组研究对象血清sTim-3和半乳糖结合蛋白9（Galectin-9）水平；双抗体夹心ELISA法检测血清中Th1型细胞因子干扰素γ（IFN-γ）和白细胞价素2（IL-2）、Th2型细胞因子IL-4和IL-10水平。结果：与健康对照组比较，URSA组患者血清sTim-3（t=7.34，P<0.05）及Galectin-9水平（t=4.86，P<0.05）均升高；URSA组患者血清sTim-3水平明显高于正常妊娠组（t=5.63，P<0.05），而Galectin-9水平则低于正常妊娠组（t=2.76，P<0.05）。正常妊娠组（t=2.81，P<0.05；t=2.84，P<0.05）和URSA组（t=3.66，P<0.05；t=2.91，P<0.05）研究对象血清IFN-γ及IL-2水平低于健康对照组；URSA组患者血清IFN-γ和IL-2水平明显高于正常妊娠组（t=6.11，P<0.05；t=5.95，P<0.05）。与健康对照组比较，正常妊娠组和URSA组研究对象血清IL-4和IL-10水平升高（t=2.53，P<0.05；t=2.52，P<0.05；t=2.69，P<0.05；t=2.33，P<0.05）；URSA组患者血清IL-4和IL-10水平明显低于正常妊娠组（t=6.48，P<0.05；t=7.76，P<0.05）。结论：sTim-3表达增加和Th1/Th2稳态调节失衡可能是导致URSA患者反复流产的关键免疫机制之一。

关键词: 可溶性Tim-3, 原因不明性复发性流产, Th1/Th2

Abstract:

Objective: To observe the changes of expression levels of serum soluble Tim-3(sTim-3) and its regulation effect on Th1/Th2 differentiaton in the patients with unexplained recurrent spontaneous abortion(URSA),and to investigate the possible immunmechanism of sTim-3 in the pathological process of URSA. Methods: A total of 52 patients with URSA were regarded as URSA group,and 20 normal pregnancy women at the same period were selected as normal pregnancy group and 20 non-pregnancy women in gestation age were selected as healthy control group.ELISA method was used to detect the levels of serum sTim-3 and galactose binding protein 9(Galectin-9) and the levels of serum Th1 cytokines interferonI-γ(IFN-γ) and IL-2 and Th2 cytokines IL-4 and IL-10 of the subjects in three groups.Results: Compared with healthy control group,the levels of serum sTim-3(t=7.34,P<0.05)and Galectin-9 (t=4.86,P<0.05) of the patients in URSA group were increased. The level of serum sTim-3 of the patients in URSA group was significantly higher than that in normal control group(t=5.63,P<0.05),but the level of serum Galectin-9 of the pathents in URSA group was lower than that in normal control group(t=2.76,P<0.05).The levels of serum IFN-γ and IL-2 of the subjects in normal control group(t=2.81,P<0.05;t=2.84,P<0.05) and URSA group(t=3.66,P<0.05;t=2.91,P<0.05) were lower than those in healthy control group.The levels of serum IFN-γ and IL-2 of the patients in URSA group were higher than those in normal control group(t=6.11,P<0.05;t=5.95,P<0.05).The levels of serum IL-4 and IL-10 of all the subjects in normal pregnancy group and URSA group were higher than those in healthy control group (t=2.53,P<0.05;t=2.52,P<0.05;t=2.69,P<0.05;t=2.33,P<0.05); the IL-4 and IL-10 levels of the patients in URSA group were lower than those in normal control group (t=6.48,P<0.05;t=7.76,P<0.05).Conclusion: The increased expression of sTim-3 and the imbalance of Th1/Th2 homeostasis may be one of the key immunomechanisms of inducing recurrent abortion in the URSA patients.

Key words: unexplained recurrent spontaneous abortion, soluble Tim-3, Th1/Th2

中图分类号:

R714.21

和秀魁, 陶莉莉, 袁晓兰, 齐一鸣, 罗喜平, 潘碧琦. 可溶性Tim-3对原因不明性复发性流产患者Th1/Th2分化的免疫调节作用及其机制[J]. 吉林大学学报(医学版), 2016, 42(06): 1163-1167.

HE Xiukui, TAO Lili, YUAN Xiaolan, QI Yiming, LUO Xiping, PAN Biqi. Immunoregulatory effectof soluble Tim-3 on Th1/Th2 differentiation in patients with unexplained recurrent spontaneous abortion and its mechanism[J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1163-1167.

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可溶性Tim-3对原因不明性复发性流产患者Th1/Th2分化的免疫调节作用及其机制

Immunoregulatory effectof soluble Tim-3 on Th1/Th2 differentiation in patients with unexplained recurrent spontaneous abortion and its mechanism

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