人参皂甙Rh2对人脑恶性胶质瘤SHG-44细胞抗肿瘤作用的蛋白质组学分析

J4 ›› 2009, Vol. 35 ›› Issue (6): 1036-1039.doi:

人参皂甙Rh2对人脑恶性胶质瘤SHG-44细胞抗肿瘤作用的蛋白质组学分析

洪新雨¹|崔佳乐²|李文臣¹|陈勃¹|罗毅男¹

1. 吉林大学第一医院神经外科|吉林长春 130021； 2.吉林大学基础医学院组织学与胚胎学教研室|吉林长春 130021

收稿日期:2009-07-22 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 罗毅男 E-mail:luo.yinan@yahoo.com.cn
作者简介:洪新雨（1977-）|男|吉林省长春市人|主治医师|医学博士|主要从事脑肿瘤的诊断和治疗研究。
基金资助:
吉林省科技厅科研基金资助课题（20010533）;吉林省中医药管理局基金资助课题（吉中医0237）

Proteomic analysis of ginsenoside-Rh2 on inhibition of human glioma cell line SHG-44

HONG Xin-yu¹|CUI Jia-yue²|LI Wen-chen¹|CHEN Bo¹|LUO Yi-nan¹

1. Department of Neurosurgery,First Hospital| Jilin University,Changchun 130021,China;2. Department of Histology and Embryology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2009-07-22 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：利用蛋白质组学研究技术分离、鉴定人脑恶性胶质瘤SHG-44细胞经人参皂甙Rh2（G-Rh2）作用后差异表达蛋白，探讨G-Rh2抗胶质瘤作用机制。方法：提取32 μmol?L-1 G-Rh2处理72 h后的SHG-44细胞及空白对照组细胞总蛋白；通过双向凝胶电泳分离蛋白质
，选取2D图谱中差异表达≥1.5倍且P<0.05的蛋白质斑点，使用基质辅助激光解析离子飞行时间质谱（MALDI-TOF-MS）进行肽指纹图谱（PMF）鉴定。结果：与空白对照组比较，SHG
-44细胞经G-Rh2作用后，双向电泳发现了20个差异蛋白质点，其中16个蛋白质点表达下调，4蛋白质点上调。质谱分析了前5个差异显著的下调蛋白质点，分别为丝切蛋白1（cofilin 1）、磷酸甘油酸激酶（phosphoglycerate kinase,PGK）、过氧化物氧化还原酶1（peroxiredoxin 1,Prx 1）、热休克蛋白（heat shock proteins，HSP）和增殖细胞核抗原（proliferation cell nuclear antigen,PCNA）。结论：差异表达的蛋白质可能参与了G-Rh2对人脑恶性胶质瘤的抗肿瘤作用。

关键词: 人参皂甙；神经胶质瘤；蛋白质组学

Abstract:

Objective
To explore the mechanism of ginsenoside-Rh2(G-Rh2) on inhibition of glioma by identifying differential proteins with proteomic technique. Methods The total proteins were extracted from SHG-44 cells treated with 32 μmol?L-1 G-Rh2 for 72 h and the cells in control group,then were subjected to two-dimensional gel electrophoresis.Only spots with a fold change equal or above 1.5 and P<0.05 were selected as differential proteins.Afterwards the differential proteins were analyzed by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for peptide mass fingerprint (PMF) identification.
Results Compared with those of control group,20 differential protein spots were identified by the two-dimensional electrophoresis in SHG-44 cells treated with G-Rh2,including 16 down-regulated ones and 4 up-regulated ones.Five remarkably down-regulated proteins analyzed by mass spectrometry were cofilin 1,phosphoglycerate kinase (PGK),peroxiredoxin 1 (Prx 1),heat shock proteins (HSP) and proliferation cell nuclear antigen (PCNA). Conclusion These differential proteins may be involved in the proliferation inhibition of human glioma cells by G-Rh2.

Key words: panaxosides;glioma;proteomics

中图分类号:

R285.5

洪新雨, 崔佳乐, 李文臣, 陈勃, 罗毅男. 人参皂甙Rh2对人脑恶性胶质瘤SHG-44细胞抗肿瘤作用的蛋白质组学分析[J]. J4, 2009, 35(6): 1036-1039.

HONG Xin-Yu, CUI Jia-Le, LI Wen-Chen, CHEN Bo, LUO Yi-Nan. Proteomic analysis of ginsenoside-Rh2 on inhibition of human glioma cell line SHG-44[J]. J4, 2009, 35(6): 1036-1039.