关键词: 钠通道, 疾病模型, 动物

Abstract: Objective To study the effect of dopamine on the epithelial sodium channel (ENaC) in conscious euvolumic rats.Methods Animals were divided into two groups: experimental group (n=29) and control group (n=16).In conscious rats,the body sodium and fluid balance were constantly maintained by servo-controlled replacements,and mean arterial blood pressure (MAP),urine volume,sodium excretion and glomerular filtration rate (GFR) were determined in response to continuous dopamine infusion (5 μg^-1•kg^-1•min^-1),and the expressions of ENaC subunits (αENaC,βENaC,and γENaC)in kidney tissues were analyzed by immunoblotting method.Results Compared with control group,the MAP and GFR in experimental group did not change(P>0.05),but urine volume and urine sodium concentration increased significantly(P<0.05);the expressions of αENaC and βENaC did not change,but the expression of γENaC was decreased(P<0.05).Conclusion Dopamine　 infusion induces a sustained natriuresis and diuresis without influence on MAP and GFR,and its mechanism may be related to down-regulation of the expression of γENaC.

Key words: sodium channels, disease models, animal