关键词: 立体定向技术, 磁共振成像, 胶质瘤, 动物模型, 大鼠, Wistar

Abstract: Objective  To establish stable and reliable rat C6 glioma model,and to perform MRI dynamic observation and pathomorphological observation in model animal brain,and to provide experimental basis for pharmaceutical research on　anti-glioma drugs.Methods The C6 glioma cells were cultured and 20 μL cultural fluid containing 1×10⁶ C6 cells was sterotactically implanted into the left caudate nuclei in 10 male Wistar rats,respectively.The changes in the behavior of the rats after implantation were observed and recorded.MRI dynamic scanning was performed in 10 rats  2,3 and 4 weeks after implantation and the brain tissues were taken for general and pathological examination when the 10 rats were naturally dead.The survival period of tumor-bearing rats was calculated.Results 2 weeks after implantation the rats showed decreased activities and food intake,fur lackluster,and conjunctival congestion a
nd so on;3 weeks later,some rats appeared nerve symptoms such as body twitch,body hemiplegy,body distortion,rotation and so on.All the 10 rats died in 8 -
30 d.The median surrival period of the tumor-bearing rats was 18 d,the average survival period was (18.3±7.3) d.The pathological examination showed that
the tumor cells were arranged irregularly and closely and karyokinesis was easy to see;tumor vascular tissue proliferation and tumor invasive growth into
surrounding noral tissues were found.The expression of glial fibrillary acidic protein(GFAP) was positive in the tumors.
Conclusion  A stable animal model of  intracranial glioma is sucessfully established by  stereotactic implantation of C6 cells into the rat caudate nucleus.The results of  MRI 〖JP2〗dynamic observation and pathohistological observation on the model animal brain tissue.can provide experimental
basis for selecting the appropriate time window to perform the pharmaceutical research on anti-glioma drugs.

Key words: stereotactic technique, magnetic resonance imaging, glioma, animal model, rat,Wistar