MMP-9及其抑制因子TIMP-1在膀胱癌中的表达及意义

doi:教育部留学回国人员科研启动基金资助课题（

MMP-9及其抑制因子TIMP-1在膀胱癌中的表达及意义

周洪澜¹，李路江¹，王医术²，王心蕊²，廖翔宇²，高婷²

1. 吉林大学第一医院泌尿外二科，吉林长春 130021；2. 吉林大学基础医学院病理生物学教育部重点实验室，吉林长春 130021

收稿日期:2006-09-03 修回日期:1900-01-01 出版日期:2007-11-28 发布日期:2007-11-28
通讯作者: 王医术

Expressions of matrix metalloproteinases-9 and its tissue inhibitor TIMP-1 in carcinoma of bladder and significances

ZHOU Hong-lan¹,LI Lu-jiang¹,WANG Yi-shu²,WANG Xin-rui²，LIAO Xiang-yu²,GAO Ting²

1.Department of Urinary Surgery,First Hospital,Jilin University,Changchun 130021,China; 2. Key Laboratory of Pathobiology,Ministryof Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2006-09-03 Revised:1900-01-01 Online:2007-11-28 Published:2007-11-28
Contact: WANG Yi-shu

摘要/Abstract

摘要： 目的：探讨基质金属蛋白酶9（MMP-9）及其抑制因子（TIMP-1）在膀胱移行细胞癌(TCCB)中的表达及意义。方法：应用免疫组织化学和RT-PCR方法检测47例TCCB组织和5例正常膀胱组织MMP-9和TIMP-1蛋白的表达情况。结果：在正常膀胱组织中MMP-9、TIMP-1不表达或呈分布均匀的微量表达，而在TCCB中则有不同程度的阳性表达，二者表达量比较差异均有显著性（P<0.05）。随着TCCB临床病理分期、分级的升高，MMP-9的表达呈现增高趋势（P<0.01），但TIMP-1表达情况与肿瘤临床病理分期、分级无明显关联（P>0.05）。MMP-9和TIMP-1在初发TCCB和复发TCCB中的表达差异均无显著性(P>0.05)。结论：MMP-9在TCCB的发生、发展中具有促进作用；MMP-9和TIMP-1之间的平衡失调可能是肿瘤侵袭和转移的分子机制之一。

关键词: 病理学, 明胶酶B, 金属蛋白酶1组织抑制剂

Abstract: Abstract:Objective To study the expressions and significances of matrix metalloproteinases-9 (MMP-9) and tissue -inhibitor of matrix metalloproteinase-1 (TIMP-1) in transitional cell carcinoma of bladder (TCCB).Methods Immunohistochemistry and RT-PCR were used to detect the expressions of MMP-9 and TIMP-1 in 47 surgical respected specimens from patients with TCCB (experimental group) and 5 normal bladder tissues (control group).Results MMP-9 and TIMP-1 had different degree expressions in experimental group,but the expressions were weak or negative in control group.Furthermore,the positive expression levels of MMP-9 were significantly increased with stages and grades (P<0.01); however the expression of TIMP-1 did not show significantly correlation with different grades and stages of TCCB tissue(P>0.05).Both MMP-9 and TIMP-1 had no significant correlation with first-occurrent TCCB and recrudescent TCCB.Conclusion MMP-9 plays advance roles in the occurrence and development of TCCB; the unbalance between MMP-9 and TIMP-1 is one of important molecular mechanisms in the aggressive and metastasis biologic behavior of tumor.

Key words: pathology, gelatinase B, tissue-inhibitor of matrix metalloproteinase-1

中图分类号:

R737.14

周洪澜，李路江，王医术，王心蕊，廖翔宇，高婷. MMP-9及其抑制因子TIMP-1在膀胱癌中的表达及意义[J]. J4, 2007, 33(6): 1057-1060.

ZHOU Hong-lan,LI Lu-jiang,WANG Yi-shu,WANG Xin-rui，LIAO Xiang-yu,GAO Ting. Expressions of matrix metalloproteinases-9 and its tissue inhibitor TIMP-1 in carcinoma of bladder and significances[J]. J4, 2007, 33(6): 1057-1060.

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