关键词: 钙粘着糖蛋白类, 牙胚, 免疫组织化学

Abstract: Abstract:Objective To observe the expressions of TGF-β1 and E-cadherin in human tooth germ development and explore the function and significance.Methods The mandible containing the tooth germs from 36 foetus bodys aged 8-34 weeks were gotten.SABC immunohistochemistry was used to detect the distribution of TGF-β1 and E-cadherin on human tooth germs.Results TGF-β1 and E-cadherin expressed in different stages of tooth development.During bud stage and cap stage,the TGF-β1 expression was positive in tooth bud epithelium,dental lamina,outer enamel epithelium and inner enamel epithelium.During bell stage,the TGF-β1 expression was strongly positive in inner enamel epithelium,enamel knot,stratum intermedium,dental lamina,odontoblast,ameloblast and positively expressed in the dental papilla cells which neared to odontoblast and dental sac.During bud stage and cap stage,the E-cadherin expression was positive in tooth bud epithelium and dental lamina.During early bell stage,the E-cadherin expression was positive in ameloblast and outer enamel epithelium.During middle bell stage,the E-cadherin expression was strongly positive in ameloblast,odontoblast and positively expressed in outer enamel epithelium and the dental papilla cells which neared to the odontoblast.During late bell stage,the expression of E-cadherin strongly positive in odontoblast,ameloblast which neared to the hard tissue and epithelial root sheath. Conclusion TGF-β1 is an important regulatory factor which participants in tooth g erm cell differentiation and morphous development.E-cadherin has close correlation with development,secretion and mineralization of enamel and dentin.

Key words: E-cadherins, tooth germ, immunohistochemistry