碱性成纤维细胞生长因子在牙发育各阶段中的表达及意义

J4 ›› 2009, Vol. 35 ›› Issue (5): 829-831.

碱性成纤维细胞生长因子在牙发育各阶段中的表达及意义

程敏¹, 刘晓红², 程琳¹, 冯志远³

1.吉林大学口腔医院预防科,吉林长春 130041；2.武警北京消防总队医院口腔科|北京　100035；3.吉林大学口腔医院正畸科|吉林长春 130041

收稿日期:2009-03-03 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 程敏 E-mail:mincheng550@sina.com
作者简介:程敏(1957-),女|吉林省长春市人|教授|主任医师|医学博士|主要从事牙发育的分子机制研究。
基金资助:
吉林省科技厅科研基金资助课题(200705350)

Expression of |basic fibroblast growth factor in each stage of tooth development and significance

CHENG Min¹, LIU Xiao-Hong², CHENG Lin¹, FENG Zhi-Yuan³

1.Department of Preventive Dentistry,Stomatology Hospital,Jilin University,Changchun 130041,China;2.Department of Stomatology,Beijing Armed Police Hospital,Fire Brigade,Beijing 100025,China;3.Department of Orthodontics,Stomatological Hospital of Jilin University,Changchun 130041,China

Received:2009-03-03 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：观察碱性成纤维细胞生长因子(bFGF)在牙发育各阶段中的表达，探讨其作用及意义。方法：以胎龄8～34周人胎儿牙胚、出生1～22 d乳鼠和出生6周～5个月犬牙为研究对象，采用免疫组织化学法定性观察bFGF发育各阶段的表达强度。结果：bFGF在牙发育的各个阶段均有表达。胎儿蕾状期和帽状牙蕾上皮、牙板、成釉器、内外釉上皮bFGF呈阳性表达，钟状期内釉细胞、中间层、成釉细胞、成牙本质细胞呈强阳性，牙乳头及牙囊bFGF呈阳性表达。乳牙萌出前、萌出期的生后乳鼠和生后6周犬牙，成釉细胞、成牙本质细胞、上皮根鞘、牙囊bFGF呈强阳性。犬恒乳牙替换期牙根、牙周膜及靠近牙周膜的成骨细胞bFGF呈阳性表达。结论：bFGF是牙发育的重要调控因子，参与牙胚细胞分化和形态发生、萌出及恒乳牙替换各阶段的调控。

关键词: 牙发育；碱性成纤维细胞生长因子；免疫组织化学

Abstract:

Abstract:Objective To observe the expression of basic fibroblast growth factor(bFGF) at different stages of tooth germ development,and explore its mechanism and significance.Methods Immunohistochemical technique was used to examine the expressions of bFGF in the tooth germs at different stages of human fetus in 8-34 weeks,the suckling mice with 1-22 d,and the dog postnatal in 6 weeks-5 months.Results bFGF expressed in tooth germ at all stages.In human fetus,the positive reaction was observed in epithelium of tooth bud，dental plate,enamel organ,external and outer enamel. The ameloblasts,odontoblasts,epithelial root sheath and dental follicle showed strongly positive reaction in the unerupted human deciduous tooth,the tooth under the eruption of neonate external enamel,stratum intermedium cells,ameloblasts and odontoblasts showed strongly positive epithelium,dental papilla and dental follicle at bud stage and cap stage,while at bell stage cells in mice and 6 week postnatal canal tooth.The positive expressions of bFGF were observed in root of tooth,peridental ligament and the osteoblasts adjoin to peridental ligament in the dog succession of decideous teeth.Conclusion bFGF is one of critical regulator in the tooth development,which can regulate the differentiation and morphogenesis of tooth germ cells and control the eruption and succession of deciduous teeth at different stages.

Key words: tooth development;basic fibroblast growth factor;immunohistochemistry

中图分类号:

R392

程敏, 刘晓红, 程琳, 冯志远. 碱性成纤维细胞生长因子在牙发育各阶段中的表达及意义[J]. J4, 2009, 35(5): 829-831.

CHENG Min, LIU Xiao-Hong, CHENG Lin, FENG Zhi-Yuan. Expression of |basic fibroblast growth factor in each stage of tooth development and significance[J]. J4, 2009, 35(5): 829-831.