关键词: 急腹症, 白细胞介素6, 肿瘤坏死因子, 一氧化氮, 脂质过氧化物, 内毒素类

Abstract: Objective To determine the changes of cytokines and inflammatory mediators in systemic inflammatory response syndrome (SIRS) in acute abdominal disease. Methods The fasting peripheral venous blood were collected in 35 patients with SIRS in acute abdominal diseases on the day of admission,and 1,3,7, and 14 days after admission, respectively. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay. The level of endotoxin in plasma was determined by tripetide azo-colorimetric method,the levels of nitric oxide (NO) and lipid peroxide (LPO) in serum were examined by fluorospectro photometry. Results The levels of TNF-α, IL-6, and endotoxin in patients were obviously higher than those in control group (P<0.01); IL-6 and TNF-α levels peaked on admission, then decreased gradually. IL-6 decreased obviously on the third and seventh days; endotoxin maintained higher level within the first 3 days on admission, but obviously lowered on the seventh and fourteenth days; serum LPO levels were high on admission, the third and seventh days (P<0.01), so were the levels of NO on admission and the third day, (P<0.05); NO and LPO maintained relatively higher levels. Conclusion There is usually infectious SIRS in acute abdominal diseases; the levels IL-6, TNF-α, NO, LPO, and endotoxin are increased in SIRS.

Key words: abdomen, acute, interleukin-6, tumor necrosis factor, nitric oxide, lipid peroxide, endotoxins