MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

doi:10.13481/j.1671-587x.20170418

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 752-756.doi: 10.13481/j.1671-587x.20170418

MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

杨竹君^1,2, 侯旭¹, 于文雯¹, 闫阔³, 申玉芹⁴, 孙新华¹

1. 吉林大学口腔医院正畸科, 吉林长春 130021;
2. 吉林省牙发育及颌骨重塑与再生重点实验室, 吉林长春 130021;
3. 吉林大学口腔医院儿童口腔科, 吉林长春 130021;
4. 吉林大学口腔医院牙周病科, 吉林长春 130021

收稿日期:2016-09-19 出版日期:2017-07-28 发布日期:2017-08-01
通讯作者: 孙新华,教授,博士研究生导师(Tel:0431-88796023,E-mail:xinhuasun8@163.com) E-mail:xinhuasun8@163.com
作者简介:杨竹君(1992-)女,陕西省蓝田县人,在读医学硕士,主要从事牙齿移动生物学方面的研究。
基金资助:
吉林省科技厅自然科学基金资助课题（20150101173JC）；吉林大学白求恩青年基金资助课题（2013208062）

Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats

YANG Zhujun^1,2, HOU Xu¹, YU Wenwen¹, YAN Kuo³, SHEN Yuqin⁴, SUN Xinhua¹

1. Department of Orthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodel, Changchun 130021, China;
3. Department of Pediatric Dentistry, Stomatology Hospital, Jilin University, Changchun 130021, China;
4. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2016-09-19 Online:2017-07-28 Published:2017-08-01

摘要/Abstract

摘要： 目的：探讨Toll样受体9（TLR9）及其下游炎症因子白细胞介素6（IL-6）在实验性大鼠牙移动过程中牙周组织中表达水平的变化，以及MT01对牙周组织TLR9、肿瘤坏死因子受体相关因子6（TRAF6）和IL-6表达水平的影响，阐明其相关机制。方法：30只雄性Wistar大鼠分为无MT01干预组（n=24）和MT01干预组（n=6）。其中，无MT01干预大鼠右侧加力为实验组，左侧不加力为对照组；MT01干预大鼠左侧上颌第一磨牙颊侧黏膜注射MT01+加力为实验组，右侧上颌第一磨牙颊侧黏膜注射PBS+加力作为对照组。0.49 N力近中移动大鼠上颌第一磨牙，无MT01干预大鼠于加力后第3、7、14和21天处死，MT01干预组于加力后第7天处死。分别获取各组大鼠上颌第一磨牙近远中侧牙槽骨，采用实时荧光定量PCR（RT-PCR）法检测张力侧和压力侧牙周组织中TLR9、TRAF6和IL-6mRNA的表达水平。结果：无MT01干预大鼠中，实验组张力侧和压力侧牙周组织中IL-6和TLR9表达水平均明显高于对照组（P<0.05），且加力7d后两者表达水平达高峰；MT01干预大鼠中，加力7d后实验组张力侧及压力侧牙周组织中TLR9表达水平较对照组降低（P<0.01），实验组压力侧牙周组织中TRAF6表达水平较对照组降低（P<0.01）。结论：MT01能够抑制实验性牙移动大鼠牙周组织中TLR9及下游相关因子TRAF6的表达，进而产生抑制牙移动过程中牙周组织炎症反应的作用。

关键词: 特定序列寡核苷酸MT01, Toll样受体9, 牙移动, 肿瘤坏死因子受体相关因子6, 白细胞介素6, 聚合酶链式反应

Abstract: Objective: To investigate the changes of expression levels of TLR9 and IL-6 in the periodontal tissue during the experimental tooth movement of the rats, and the effecst of the MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue,and to clarify its related mechanisms.Methods: Thirty Wistar rats were randomly divided into MT01 intervention group(n=6) and non-MT01 group(n=24). Force of 0.49 N was applied to move the upper first molars mesially. The rats in Non-MT01 intervention group were sacrificed on the days 3,7,14 and 21, and the rats in MT01 intervention group were all sacrificed on the day 7. RT-qPCR was used to detect the expression levels of TLR9,TRAF6 and IL-6 mRNA in maxillary first molar alveolar bone in each group.Results: The expression levels of IL-6 and TLR9 mRNA in loaded side were higher than those in control side(P<0.05), and reached the maximum levels on the day 7(P<0.01); with the interpose of MT01, the expression levels of TLR9 and TRAF6 mRNA were lower than control side(P<0.01).Conclusion: MT01 could down-regulate the expression levels of TLR9 and TRAF6 during orthodontic tooth movement and eventually resists the inflammation during the tooth movement.

Key words: MT01, tooth movement, tumor necrosis factor receptor associated factor 6, toll like receptors 9, interleukin-6, polymerase chain reaction

中图分类号:

R783.5

杨竹君, 侯旭, 于文雯, 闫阔, 申玉芹, 孙新华. MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响[J]. 吉林大学学报(医学版), 2017, 43(04): 752-756.

YANG Zhujun, HOU Xu, YU Wenwen, YAN Kuo, SHEN Yuqin, SUN Xinhua. Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats[J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 752-756.

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MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats

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