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• 基础研究 • 上一篇    下一篇

恒定磁场对老龄小鼠脑、心、肾脂褐素及心、肾、胸腺抗氧化酶含量的影响

付 妍1,梁路光1,姚树仁2,李晓林3,李晓梅3,陆艳娟3   

  1. 1.吉林大学物理教学中心,吉林 长春 130021;2.吉林大学基础医学院解剖学教研室,吉林 长春 130021;3. 吉林大学基础医学院生物化学教研室,吉林 长春130021
  • 收稿日期:2004-02-23 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28

Influences of stationary magnetic fields on contents of LPF in brain,heart ,kidney, and GSH-Px in heart, kidney, and thymus of old mice

FU Yan1,LIANG Lu-guang1,YAO Shu-ren2,LI Xiao-lin3,LI Xiao-mei3,LU Yan-juan3   

  1. 1.Physics Teaching Center, Jilin University,Changchun 130021,China;2. Department of Anatamy, School of Basic Medical Sciences,Jilin University, Changchun 130021,China; 3.Departmentof Biochemistry, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
  • Received:2004-02-23 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28

摘要: 目的:研究恒定磁场对老龄小鼠脑、心、肾组织脂褐素(LPF)及心、肾、胸腺组织谷胱甘肽过氧化物酶(GSH-Px)含量的影响。 方法:将20只雌性老龄小鼠随机分为实验组(n=10)与对照组(n=10)。把实验组老龄小鼠放入磁场强度为200 mT的铁箱中,每日曝磁1 h,连续30 d。 结果:实验组老龄小鼠脑组织脂褐素含量[(0.160±0.024)μg•g-1]较对照组[(0.304±0.061)μg•g-1]比较差异有显著性(P<0.05);心、肾组织脂褐素含量[(0.082±0.009)及(0.329±0.024)μg•g-1]与对照组[(0.092±0.011)及(0.382±0.033)μg•g-1 ]比较,差异无显著性(P>0.05)。实验组心、肾及胸腺GSH-Px活性[(9.63±1.52)、(30.68±4.50)及(96.72±10.94)NU•mL-1]较对照组[(7.63±1.48)、(24.09±4.49)及(85.68±7.12)NU•mL-1]明显升高(P<0.05及P<0.01)。 结论:磁场强度200 mT的恒定磁场有降低老龄小鼠器官组织中脂褐素含量、抵抗自由基及提高抗氧化酶活性的作用。

关键词: 衰老, 代谢, 谷胱甘肽过氧化酶, 脂褐素, 自由基

Abstract: Objective To study the influences of stationary magnetic fields on the contents of lipofuscin (LPF) in brain,heart, and kidney and the contents of GSH-Px in heart,kidney, and thymus of old mice. Methods Twenty female old mice were randomly divided into experimental group and control group. The mice in the experimental group were then put into a metal box where magnetic induction was 200 mT. They were exposed to the magnetic field one hour every day for 30 days running. Results The content of LPF in brain of old mice in experimental group ((0.160±0.024) μg•g-1) was reduced remarkably (Ρ<0.05), compared with the control group ((0.304±0.061) μg•g-1). But there were no significant differences of the contents of LPF in heart and kidney ((0.082±0.009) and (0.329± 0.024) μg•g-1) (P>0.05) in experimental group, compared with the control group((0.092±0.011)and (0.382±0.033) μg•g-1). The activities of GSH-Px in heart, kidney, and thymus of old mice in experimental group ((9.63±1.52),(30.68±4.50),and (96.72±10.94) NU•mL-1) were increased remarkably (P<0.05 or P<0.01), compared with control group ((7.63±1.48),(24.09±4.49),and (85.68±7.122) NU•mL-1). Conclusion In the stationary magnetic fields (20 mT), the contents of LPF in organs are reduced, free radicals are resisted and the activity of anti-oxide ferment is increased.

Key words: aging, metabolish, glutathione peroxidase, lipofuscin, free radicals

中图分类号: 

  • R-33