吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (02): 288-292.doi: 10.13481/j.1671-587x.20170215

• 基础研究 • 上一篇    下一篇

鸢尾黄素对大鼠心肌纤维化的作用及其机制

赵丽晶1, 康晶2, 安英1, 徐博1, 王艳春2   

  1. 1. 吉林医药学院基础医学院机能教研室, 吉林 吉林 132013;
    2. 吉林医药学院教务处, 吉林 吉林 132013
  • 收稿日期:2016-02-25 出版日期:2017-03-28 发布日期:2017-03-31
  • 通讯作者: 王艳春,教授,硕士研究生导师(Tel:0432-64560186,E-mail:wangyanchun1972@163.com) E-mail:wangyanchun1972@163.com
  • 作者简介:赵丽晶(1971-),女,吉林省公主岭市人,副主任医师,医学硕士,主要从事心血管疾病药理学方面的研究。
  • 基金资助:
    吉林省教育厅"十二五"科学技术研究项目资助课题(2015396)

Eeffect of tectorigenin on myocardial fibrosis in rats and its mechanism

ZHAO Lijing1, KANG Jing2, AN Ying1, XU Bo1, WANG Yanchun2   

  1. 1. Medical Functional Laboratory, School of Basic Medical Sciences, Jilin Medical College, Jilin 132013, China;
    2. Office of Educational Administration, Jilin Medical College, Jilin 132013, China
  • Received:2016-02-25 Online:2017-03-28 Published:2017-03-31

摘要: 目的:探讨鸢尾黄素对大鼠心肌纤维化(MF)的作用并阐明其作用机制,为临床用药提供参考。方法:60只Wistar大鼠随机分为正常对照组、模型组、阳性药(卡托普利)对照组和低、中、高剂量鸢尾黄素组,每组10只。除正常对照组外,其余各组大鼠采用异丙肾上腺素(Iso)皮下注射(5 mg·kg-1·d-1),连续7d,构建大鼠MF模型。除正常对照组和模型组外,其他各组于造模第2天灌胃给药,分别给予25、50、100 mg·kg-1·d-1鸢尾黄素和10 mg·kg-1·d-1卡托普利,连续28d。实验结束后采用BL-420 E+生物机能实验系统检测大鼠心功能,测量心质量指数(HMI)和左心室质量指数(LVMI),紫外分光法检测心肌组织中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性,酶标仪检测血清中乳酸脱氢酶(LDH)和肌酸激酶(CK)活性及一氧化氮(NO)水平,ELISA法检测血清中Ⅰ型胶原(ColⅠ)和Ⅲ型胶原(Col Ⅲ)水平,HE染色观察各组大鼠心肌组织病理表现。结果:与正常对照组比较,模型组大鼠心率(HR)加快、左室收缩压(LVSP)降低(P<0.01);HMI和LVMI升高(P<0.05);左心室心肌组织中MDA水平升高(P<0.01),SOD活性降低(P<0.01);血清中ColⅠ和Col Ⅲ水平升高,LDH和CK活性升高(P<0.01);而NO水平降低(P<0.01)。与模型组比较,各鸢尾黄素组大鼠HR减慢(P<0.05或P<0.01),LVSP升高(P<0.05或P<0.01);HMI和LVMI均降低;心肌组织中MDA水平降低(P<0.05或P<0.01),SOD活性升高(P<0.05或P<0.01);血清中ColⅠ、Col Ⅲ水平和CK活性均降低(P<0.05或P<0.01);中、高剂量组LDH活性明显降低(P<0.01),各剂量组NO水平升高(P<0.05或P<0.01)。结论:鸢尾黄素可抑制Iso所致的大鼠MF,其机制可能与抗氧化、清除自由基进而抑制胶原的合成有关。

关键词: 鸢尾黄素, 异丙肾上腺素, 胶原, 心肌纤维化, 自由基

Abstract: Objective: To explore the effect of tectorigenin on myocardial fibrosis(MF) in the rats and clarify the related mechanism,and to provide reference for its clinical application. Methods: Sixty Wistar rats were randomly divided into normal control group,model group,positive drug (capropril) control group, and low,middle,high doses of tectorigenin groups(n=10).Except normal control group, the rats in other groups were used to construct MF models by subcutaneous injection of 5 mg·kg -1·d -1 isoproterenol (Iso) for 7 d.The rats in tectorigenin groups and captopril group were intragastricly administrated with different doses of tectorigenin (25,50,100 mg·kg-1·d-1)and captopril(10 mg·kg -1·d -1) from the second day after modeling for consecutive 28 d.Bl-420E+ biological function experiment system was used to detect the heart function; Heart mass index (HMI) and left ventricular mass index (LVMI) were measured after experiment.UV detection was used to measure the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) in myocardial tissue.Microplate reader was used to measure the activities of lactic dehydrogenase(LDH) and creatine kinase(CK) and the levels of nitric oxide (NO)in serum.ELISA were used to detect the levels of collagen typeⅠ(ColⅠ) and collagen type Ⅲ (Col Ⅲ) in myocardium tissue of the rats.The pathological changes of myocardium tissue of the rats in various groups were observed by HE staining. Results: Compared with normal control group,the HR of rats in model groups was increased,and the left ventricular systolic pressure (LVSP) was decreased(P<0.01); the HMI and LVMI were increased(P<0.05), the levels of MDA in left ventricular myocardial tissue was increased(P<0.01), and the activity of SOD was decreased,the levels of serum ColⅠ,Col Ⅲ and the activities of LDH, CK were also increased(P<0.01);the level of NO in serum was decreased(P<0.01).Compared with model groups, the HR were decreased,LVSP were increased, and HMI and LVMI of the rats in different doses of tectorigenin groups were decreased in a dose-dependent manner; the levels of MDA were reduced;the activities of SOD were increased in myocardium tissue,and the CK activities and the ColⅠ and ColⅢ levels were decreased(P<0.05 or P<0.01);the LDH activities in middle and high doses of tectorgenin groups were decreased(P<0.01); and the levels of NO in serum in different doses of tectorigenin groups were significantlyincreased(P<0.05 or P<0.01). Conclusion: Tectorigenin could inhibit the MF induced by Iso in the rats, and its mechanism may be related to antioxidation,scavenging free radical and inhibition of collagen synthesis.

Key words: myocardial fibrosis, isoproterenol, collagen, tectorigenin, free radical

中图分类号: 

  • R542.23