COX-2基因在人脑星形细胞肿瘤组织中表达及其与Fas基因表达的相关性

doi:辽宁省科技厅自然科学基金资助课题（200310

COX-2基因在人脑星形细胞肿瘤组织中表达及其与Fas基因表达的相关性

冯天达，于宏伟，刘云会，潘蔚然，刁宏宇，王成林

中国医科大学附属第二医院神经外科，辽宁　沈阳110004

收稿日期:2006-09-08 修回日期:1900-01-01 出版日期:2007-03-28 发布日期:2007-03-28

Expression of cyclooxygenase-2 mRNA in human astrocytic tumors and its correlation with Fas mRNA expression

FENG Tian-da, YU Hong-wei, LIU Yun-hui, PAN Wei-ran,　DIAO Hong-yu, WANG Cheng-lin

Department of Neurosurgery,Second Affiliated Hospital,China Medical University, Shenyang 110004, China

Received:2006-09-08 Revised:1900-01-01 Online:2007-03-28 Published:2007-03-28

摘要/Abstract

摘要： 目的：探讨COX-2 mRNA在星形细胞肿瘤组织中的表达及与Fas mRNA表达的相关性。方法：应用RT-PCR技术检测不同级别星形细胞肿瘤组织及非肿瘤组织中COX-2 mRNA和Fas mRNA的表达水平。结果：COX-2 mRNA表达阳性率分别为：非肿瘤组织33.3%，肿瘤组织78.4%；Fas mRNA阳性表达率分别为：非肿瘤组织8.3%，肿瘤组织74.5%；二者在肿瘤组织中的表达显著高于其在非肿瘤组织中的表达（P<0.05），且有良好的相关性（r=0.525，P=0.008）。结论：COX-2 mRNA表达与星形细胞肿瘤的发生、发展有关，是评估星形细胞肿瘤恶性生物学行为的可靠指标；COX-2 mRNA表达与Fas mRNA密切相关，两者可能存在共同的激活机制，构成抑制肿瘤细胞凋亡的多种途径。

关键词: 环氧合酶-2, Fas基因, 逆转录聚合酶链反应

Abstract: Objective To detect the expression of COX-2 mRNA in astrocytic tumors (ATs) tissue, also to analyze its correlation with the expression of Fas mRNA. MethodsReverse transcription polymerase chain reaction (RT-PCR) technique was used to examine the levels of expression of COX-2 mRNA and Fas mRNA in ATs tissue and non-tumor tissue.Results The rates of of COX-2 mRNA in non-tumor tissue and ATs tissue were 33.3%and 78.4%, respectively. The positive exprssion rates of Fas mRNA in non-tumor tissue and ATs tissue were 8.3% and 74.5%. The expression levels of COX-2 mRNA and Fas mRNA in ATs tissue were significantly higher than those in non-tumor tissue（P<0.05）. There was obvious correlation between expressions of COX-2 mRNA and Fas mRNA（r=0.525,P=0.008）. Conclusion The expression of COX-2 mRNA is correlated with carcinogensis and development of ATs, and it is an effective marker in diagnosis of ATs. A positive correlation between expressions of COX-2 mRNA and Fas mRNA is found, it is speculated that there may be a common transcription mechanism which constructs multiple pathways contributing to the inhibition of apoptosis in ATs.

Key words: cyclooxygenase-2, Fas, reverse transcription polymerase chain reaction

中图分类号:

R739.41

冯天达，于宏伟，刘云会，潘蔚然，刁宏宇，王成林. COX-2基因在人脑星形细胞肿瘤组织中表达及其与Fas基因表达的相关性[J]. J4, 2007, 33(2): 321-323.

FENG Tian-da, YU Hong-wei, LIU Yun-hui, PAN Wei-ran,　DIAO Hong-yu, WANG Cheng-lin. Expression of cyclooxygenase-2 mRNA in human astrocytic tumors and its correlation with Fas mRNA expression[J]. J4, 2007, 33(2): 321-323.

[1]	董泽丰, 郑田利, 范雪佳, 裴晓方. 2011-2013年成都地区发热呼吸道症候群住院患儿鼻病毒流行病学特征[J]. 吉林大学学报(医学版), 2016, 42(01): 177-181.
[2]	孙勤暖，李冬梅，于慧玲，马洪喜. 甲状腺乳头状癌组织中炎性因子的表达及其临床意义[J]. 吉林大学学报(医学版), 2014, 40(06): 1261-1265.
[3]	方艳秋，齐亚灵，芦小单，马寅芙，谭岩. 环氧合酶2抑制剂NS-398对人肝癌细胞SMMC-7721增殖与凋亡的影响[J]. 吉林大学学报(医学版), 2013, 39(6): 1190-1194.
[4]	刘洁, 贾天军, 刘雪晴, 贾晓辉, 翟雪琼. 人甘露糖结合凝集素相关丝氨酸蛋白激酶-2 C端基因的克隆与鉴定[J]. J4, 2011, 37(1): 51-55.
[5]	宋明旭, 李莉华, 丁苏宁, 刘志辉, 周希科. PDGF家族在原发性胃腺癌组织中的表达及其意义[J]. J4, 2011, 37(1): 97-101.
[6]	张晓霞, 孙晓琦, 张松灵, 高咏梅, 田海涵. 卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义[J]. J4, 2010, 36(3): 536-539.
[7]	张宸豪，柳忠辉，刘海岩，王轶楠，葛敬岩，于昉，台桂香. 激活素A及其结合蛋白在STZ损伤小鼠胰腺组织中的表达及意义[J]. J4, 2009, 35(2): 292-295.
[8]	张晓霞，费军伟，舒畅，倪劲松，王芯蕊，李荷莲. 卵巢浆液性囊腺癌组织中prostasin基因的表达及意义[J]. J4, 2009, 35(2): 280-283.
[9]	汲坤，王波，李馨，于浩，张灵，胡嘉弟，赵雪俭，李扬. 细胞死亡调节因子GRIM-19蛋白在肾脏透明细胞癌组织中的表达及意义[J]. J4, 2009, 35(1): 146-149.
[10]	马舒贝，孙威，李霞，刘亢丁. 大鼠局灶性脑缺血后脑组织Ang-1和Ang-2 mRNA的表达及意义[J]. J4, 2009, 35(1): 30-33.
[11]	张晓霞，费军伟，于晓伟，倪劲松，王芯蕊，李荷莲. 卵巢癌组织中PI3K的表达及意义[J]. J4, 2008, 34(6): 1027-1030.
[12]	李光虎，刘伟，付彤，刘国津. muc-1基因在非小细胞肺癌组织中的表达及临床意义[J]. J4, 2008, 34(6): 1046-1049.
[13]	李玉琴，黄民，王英凯，金京兰，唐彤宇，高沿航，王丹. 热休克蛋白70在消化性溃疡及急性糜烂出血性胃炎组织中的表达及意义[J]. J4, 2008, 34(5): 846-849.
[14]	牟旭鹏，孔宁，申茉函，韩东，张林，颜炜群. 人碱性成纤维细胞生长因子cDNA的克隆及在毕赤酵母中的表达[J]. J4, 2008, 34(5): 755-758.
[15]	方艳秋，齐亚灵，刘婷，段秀梅，谭岩，陈东. COX-2和VEGF蛋白在HCC组织中表达及其与HCC血管形成的关系 [J]. J4, 2008, 34(5): 864-866.