关键词: 缺血预处理, 脑缺血, 再灌注损伤, caspase-3

Abstract: To investigate the protective effect of penehyclidine hydrochloride pretreatment on focal cerebral ischemia/reperfusion injury and expression of caspase-3 in rats.Methods Sixty male Sprague-Dawley rats weighing 250-300 g were randomly divided into 3 groups(n=20): control （C） group in which sham operation was performed; ischemia-reperfusion （I-R）group, in which saline （2.0 mg•kg^-1）was administered for intraperitoneal injection 30 min before anesthesia,then the ischemia model was made by occlusion of the left middle cerebral artery for 120 min; penehyclidine hydrochloride pretreatment （P） group, in which penehyclidine hydrochloride（2.0 mg•kg^-1）was administered for intraperitoneal injection 30 min before anesthesia,then the same 120 min focal cerebral ischemia was performed as described in I-R group.At the end of 24 h reperfusion the animals were decapitated and the brains were removed.The volume of cerebral infarction was detected with TTC staining;Coronal sections including hippocampal tissue were obtained for HE staining.The levels of caspase-3 mRNA expression in hippocampus were detected by using semi-quantitative RT-PCR technique. Results Compared with group I-R，the volume of cerebral infarction in group P were reduced significantly（P< 0.05）;In group P,edema,degeneration and necrosis of nerve cells in the hippocampus of the rats were significantly decreased; The expressions of caspase-3 mRNA in group I-R and group P increased significantly compared with group C（P< 0.05）,and the caspase-3 mRNA expression in group P was lower than that in group I-R（P< 0.05）. Conclusion Penehyclidine hydrochloride has brain protective effect on focal cerebral ischemia/reperfusion injury in rats and its mechanism is related to inhibiting the expression of caspase-3 mRNA in hippocampus.

Key words: ischemic preconditioning, brain ischemia, reperfusion injury, caspase-3