口服供者脾细胞诱导成年大鼠心脏移植存活期延长

doi:全军医学科研“十五”计划重大项目基金资助

口服供者脾细胞诱导成年大鼠心脏移植存活期延长

佟建秋¹，石炳毅²，王禾¹，沈瑞雄¹，莫春柏²，周文强²

1. 第四军医大学西京医院泌尿外科，陕西西安 710032;2. 解放军第309医院泌尿外科，北京 100091

收稿日期:2002-10-12 修回日期:1900-01-01 出版日期:2004-03-28 发布日期:2004-03-28

Prolongation of survival period of heart allograftsin adult rats by feeding of donor spleen cells

TONG Jian-qiu¹, SHI Bing-yi², WANG　He¹，SHEN Rui-xiong¹,MO Chun-bai²,ZHOU Wen-qiang²

1. Department of Urology,Xijing Hospital,Fourth Military Medical University, Xi′an 710032,China;2. Department of Urology,No.309 Hospital of PLA,Beijing 100091, China

Received:2002-10-12 Revised:1900-01-01 Online:2004-03-28 Published:2004-03-28

摘要/Abstract

摘要： 目的:利用口服供者脾细胞延长受者大鼠的心脏移植存活时间，并探讨口服耐受的形成机制。方法:以纯系SD大鼠为供者，纯系Wistar大鼠为受者，行异体心脏移植，将16只受者大鼠随机分为A组(对照组，单纯行心脏移植)和B组(口服抗原组)，于心脏移植前7 d开始每日接受供者脾细胞5×10⁷个，导管灌胃，7 d后观察体外混合淋巴细胞培养(MLC)、体内的迟发型超敏反应(DTH)，并行心脏移植，观察移植心脏的存活时间。结果:口服抗原后，体外混合淋巴细胞培养及体内的DTH反应均出现明显的抗原特异性降低，口服抗原组的心脏移植存活时间达到(12.1±1.9)d，而对照组为(7.0±0.8)d，两组比较差异有显著性(P<0.01)。结论:口服抗原可以引起受者对异基因抗原的特异性免疫反应降低，使受者的心脏移植存活期延长。

关键词: 心脏移植, 细胞培养, 超敏反应, 迟发型

Abstract: Objective To induce the prolongation of survival period of heart allografts in recipient rats by oral administration of donor spleen cells,and to study the mechanism of oral tolerization. Methods The inbred SD rats were chosen as heart donors and inbred Wistar rats as recipients,heterotopic heart transplantations were performed. Sixteen recipients were divided into group A (control group,only performing heterotopic heart transplantation) and group B(feeding group,feeding on 5×10^7 donor splenocytes by means of gastric intubation each day). After 7 days, the mixed lymphocyte culture (MLC) in vitro and delayed-type hypersensitivity response (DTH) in vivo were examined, and the allogenic heart transplantation was performed, then the survival time of heart allograft was observed.　 Results MLC in vitro and DTH in vivo existed antigen specific reduction after rats were fed on alloantigen orally. The survival period of heart allografts in group A and group B were (7.0±0.8)d and (12.1±1.9)d ,respectively. Conclusion Oral administration of alloantigen can down-regulate the immune response to histocompatibility antigens, prolong the survival times of heart allografts.

Key words: heart transplantation, cell culture, hypersensitivity, delayed

中图分类号:

R392.4

佟建秋，石炳毅，王禾，沈瑞雄，莫春柏，周文强. 口服供者脾细胞诱导成年大鼠心脏移植存活期延长[J]. J4, 2004, 30(2): 215-217.

TONG Jian-qiu, SHI Bing-yi, WANG　He，SHEN Rui-xiong,MO Chun-bai,ZHOU Wen-qiang. Prolongation of survival period of heart allograftsin adult rats by feeding of donor spleen cells[J]. J4, 2004, 30(2): 215-217.

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