当归红芪超滤物对心肌细胞Hsp70表达的影响

J4 ›› 2010, Vol. 36 ›› Issue (1): 5-9.

当归红芪超滤物对心肌细胞Hsp70表达的影响

马燕花^1,2|李应东²|赵健雄¹

（1.兰州大学中西医结合研究所|甘肃兰州 730000；2.甘肃中医学院临床医学系|甘肃兰州 730000）

收稿日期:2009-04-28 出版日期:2010-01-28 发布日期:2010-01-28
通讯作者: 李应东 E-mail:(Tel:0931-8635068,E-mail:lydj412@163.com）
作者简介:马燕花（1976-）|女|甘肃省兰州市人|讲师|主治医师|在读医学博士|主要从事中西医结合内科学基础及临床研究。
基金资助:
“十一五”国家科技支撑计划项目子课题（2006BAI06A20-04）；国家科技
支撑计划项目资助课题（2007BAI37B01）

Effect of ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on expression of Hsp70 in cardiomyocytes

MA Yan-Hua^1,2, LI Ying-Dong², DIAO Jian-Xiong²

(1.Institute of Combination of Chinese Traditional with Western Medicine |Lanzhou University|

Received:2009-04-28 Online:2010-01-28 Published:2010-01-28

摘要/Abstract

摘要：

目的：探讨当归红芪超滤物对凋亡心肌细胞热休克蛋白70（Hsp70）表达的影响，阐明当归红芪超滤物对心肌细胞的抗氧化作用。方法：原代培养的Wistar乳鼠心肌细胞用高浓度的H₂O₂（400 μmol•L^-1）建立细胞凋亡模型，用不同浓度（3.75、7.50和15.00　g•L^-1）的当归红芪超滤物进行干预，倒置显微镜下观察各实验组心肌细胞搏动频率，流式细胞仪检测心肌细胞凋亡率，分别用RT-PCR技术和Western blotting技术检测心肌细胞中Hsp70基因及蛋白的表达情况。结果：与对照组比较，H2O2损伤组心肌细胞搏动频率显著降低(P<0.01)，细胞凋亡率显著增加(P<0.01)，Hsp70 mRNA及蛋白表达量升高 (P<0.05，P<0.01)；与H₂O₂损伤组比较，当归红芪超滤物高、中、低剂量组心肌细胞搏动频率显著增加，但低于对照组(P<0.01)，凋亡率显著降低(P<0.01)，Hsp70 mRNA及蛋白表达量显著升高（P<0.05，P<0.01）。结论：当归红芪超滤物具有抗氧化损伤心肌细胞凋亡的作用，且可上调心肌细胞Hsp70的表达。

关键词: 当归红芪超滤物；细胞凋亡；肌细胞, 心脏;HSP热休克蛋白质类
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Abstract:

Abstract:Objective
To investigate the effect of the ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on heat shock protein 70(Hsp70)expression in cardiomyocytes,and clarify its antioxidation on cardiomyocytes．Methods Myocardial cells from 1-3 d neonatal rats were cultivated in DF medium and the cellular injury was induced by H₂O₂（400 μmol•L^-1）．The ultra-filtration extract mixtures were given in three doses of 3.75，7.50， and 15.00 g•L^-1．The beating rates of cardiomyocytes were observed by phase-contrast microscope.The apoptotic rates of cardiomyocytes were measured by flow cytometry．The expressions of Hsp70 mRNA and protein in cardiomyocytes were measured by RT-PCR and Western blotting．Results Compared with normal control group,the beating rates of cardiomyocytes were significantly decreased (P<0.01) and the apoptotic rates of cardiomyocytes were significantly increased(P<0.01) in model group,and the expressions of Hsp70 mRNA and protein in cardiomyocytes were increased(P<0.05，P<0.01).Compared with model group,the beating rates of cardiomyocytes were significantly increased(P<0.01) and the apoptotic rates were significantly decreased (P<0.01) in each treatment group,and the expressions of Hsp70 mRNA and protein in cardiomyocytes were significantly increased（P<0.01，P<0.05）.Conclusion The ultra-filtration extract mixture has the protective effects on cardiomyocytes injured by H₂O₂and can up-regulate the Hsp70 expression in cardiomyocytes．

Key words: ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys；apoptosis；myocytes,cardiac；HSP70 heat-shock proteins

中图分类号:

马燕花, 李应东, 赵健雄. 当归红芪超滤物对心肌细胞Hsp70表达的影响[J]. J4, 2010, 36(1): 5-9.

MA Yan-Hua, LI Ying-Dong, DIAO Jian-Xiong. Effect of ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on expression of Hsp70 in cardiomyocytes[J]. J4, 2010, 36(1): 5-9.