关键词: K562细胞, NB4细胞, 细胞增殖, 细胞凋亡

Abstract: To study the effect of methylmercury chloride(MMC) on proliferation and apoptosis in leukemia cell lines NB4 and K562. Methods NB4 and K562 were treated with 1.25,2.50,5.00,10.00 and 20.00 μmol•L^-1 MMC and asenic troxide (ATO) for 0,6,12,24,48 and 72 h,the cell viability rate was detected by MTT assay; the apoptosis was observed with fluorescence microscope after stained by Hoechst 33258. Results The viability rates of NB4 and K562 cells treated with 10 μmol•L^-1 MMC for 24 h were 22.0% and 70.0%；when treated with 10　μmol•L^-1 ATO for 24 h the viability rates were 24.4 % and 52.0%; compared with control group,there was significant difference (P<0.05). The significant apoptostic picture was obtained with fluoresce nce microscope. The two drugs had the similar effect when they were used to treat NB4 and K562 cells in the same dose and for the same time. Conclusion MMC can induce apopt osis of NB4 and K562 cells and inhibit proliferation of leukemia cell lines NB4 and K562 significantly and the effect is in time- and dose-dependent manner.

Key words: K562 cells, NB4 cells, proliferation, apoptosis