关键词: 有丝分裂, suramin, 生长和胚 胎发育

Abstract: Objective To evaluate the biological effect of recombinant acidic fibroblast growth factor (raFGF) with nuclear traslocation sequence (NLS) knocked-out through comparing the effects of waFGF, raFGF and suramin on growth and development in new-born rats. Methods Wistar new-born rats were divided into 5 groups, namely normal, blank, suramin, suramin+waFGF (s+w) and suramin+raFGF (s+r), respectively. The differences of body weight (BW), outlook, survival rate and time of opening eyes in new-born rats were observed. Results The BW in s+r group at 16 d after birth of rats was significantly lower than that in s+w group. However, the BW in s+r and s+w groups were significantly lower than those in normal and blank groups. There wer e the desquamate symptoms in the back of new-born rats in suramin and s+r groups and the opisthotonos in suramin and s+r groups with different degrees. All the rats in suramin group had not opened their eyes till death at 16 d after birth. In normal, blank, s+w and s+r groups, the times of opening eyes in new-born rats were 14, 4, 19 and 22 d after birth, respectively. Their survival rates were 99.3％, 87.6％, 66.7% and 20.0%，respectively, within 22 d after birth, and there was a significant difference between s+w and s+r groups (P<0.05). Conclusion Acting as the antagonist of aFGF, suramin can inhibit body′s growth and development, this inhibition can be turned over by exogeneous aFGF to some extent,and raFGF has no such significant effect. It suggests that the aFGF with NLS knocked-out affects new-born rats slightly and may lie in its decreased mitogenic activity.

Key words: mitosis, suramin, growth and embryonic development