新型TaqMan-MB荧光探针测定古DNA模板含量

doi:国家基础学科人才培养基金资助课题(J530184

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新型TaqMan-MB荧光探针测定古DNA模板含量

崔银秋^1,2，张全超¹，谢承志¹，周慧¹,

1.吉林大学边疆考古研究中心古DNA实验室，吉林长春　130012；2.吉林大学生命科学学院大分子实验室，吉林长春 130023

收稿日期:2006-02-21 修回日期:1900-01-01 出版日期:2007-01-28 发布日期:2007-01-28
通讯作者: 周慧

New type taqman-MB of fluorogenic probe for ancient DNA template detection

CUI Yin-qiu^1,2, ZHANG Quan-chao¹, XIE Cheng-zhi¹,ZHOU Hui^1,2

1. Ancient DNA Laboratory, Research Center for Chinese Frontier Archaeology, Jilin University, Changchun 130012,China；2. Macromolecular Laboratory, College of Life Science, Jilin University, Changchun 130023,China

Received:2006-02-21 Revised:1900-01-01 Online:2007-01-28 Published:2007-01-28
Contact: ZHOU Hui

摘要/Abstract

摘要： 目的:设计荧光检测探针，对古DNA样本中的线粒体DNA片段进行定量分析，并对其检测效果进行评价。方法:利用分子信标的茎环结构和TaqMan的降解作用设计一种新型的TaqMan-Molecular Beacon荧光检测探针，与实时PCR仪联用，对古DNA模板进行定量检测。结果: 5个古代样本中3个测得拷贝数，1个无法观察到原始模板量，1个样本无原始模板。结论: TaqMan-MB荧光探针集中了分子信标和TaqMan的技术优势，对古DNA模板具有很好的定量检测效果。

关键词: 实时定量PCR, 定量分析

Abstract: Abstract:ObjectiveTo develop a fluorogenic probe for measuring the concentration of DNA extracted from ancient samples，and evaluate its detection effects . Methods A novel fluorogenic probe, TaqMan-Molecular Beacon (TaqMan-MB), which combined the hairpin structure of molecular beacons and the cleavage mechanism of TaqMan probes，was developed. Combined with a real-time PCR instrument, TagMan-MB was used for quantification of templates of ancient DNA. ResultsIn all five ancient samples, three samples were gotten the copy numbers, one sample had no template, and one sample cannot be detected. Conclusion TaqMan-MB has much better detection effects than TaqMan or molecular beacons alone, and presents a promising approach for quantification of target genes.

Key words: real-time PCR, quantification

中图分类号:

Q987

崔银秋，张全超，谢承志，周慧,. 新型TaqMan-MB荧光探针测定古DNA模板含量[J]. J4, 2007, 33(1): 9-12.

CUI Yin-qiu, ZHANG Quan-chao, XIE Cheng-zhi,ZHOU Hui. New type taqman-MB of fluorogenic probe for ancient DNA template detection[J]. J4, 2007, 33(1): 9-12.

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新型TaqMan-MB荧光探针测定古DNA模板含量

New type taqman-MB of fluorogenic probe for ancient DNA template detection

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