关键词: 细胞凋亡, 高浓度葡萄糖, NF-κB, 细胞色素C类

Abstract: Abstract:Objective To study the role of NF-kappaB( NF-κB) on islet cell apoptosis in high concentration glucose in vitro.Methods Pancreatic islet cells wereisolated from Kunming mice and these cells were cultivated in medium with different concerntrations of glucose: G1 (5.6 mmol•L^-1),G2(7.8 mmol•L^-1),G3(11.1 mmol•L^-1),G4(16.7 mmol•L^-1),G5(22.2 mmol•L^-1),G6(27.6 mmol•L^-1).After the slet cells were cultivated for 72 h,insulin secretion was evaluated by radio-immunity technique.The NF-κB expression was detected by immunocytochemistry and cytochrome C(Cyt.C) release was detected by immunofluorescence technique.Apoptosis of islet cells was determined by Hoechst33342 assay. Results Insulin secretion was enhanced and NF-κB activity was increased（P<0.05）when the concentrations of glucose were 5.6-11.1 mmol•L^-1,but Cyt.C release and apoptosis didn’t change. When the concentration of glucose exceeded 16.7 mmol•L^-1,the apoptotic percentages of islet cells were increased signficantly accompanying with the glucose concentration elevation compared with G₁,G₂ and G₃ groups(P<0.05),the NF-κB activity and Cyt.C relesae were significantly increased （P<0.05）.Conclusion High glucose can induce the apoptosis of islet cells and decrease insulin secretion,enhance the activity of NF-κB and Cyt C release.It suggests that the activation of NF-κB may be related to apoptosis of islet cells,and the inhibition of NF-κB may protect islet cells.

Key words: apoptosis, high concentration glucose, NF-kappa B, cytochrome C