脑红蛋白在脑梗死大鼠脑组织中的表达及其神经保护作用机制

J4 ›› 2012, Vol. 38 ›› Issue (6): 1114-1118.

脑红蛋白在脑梗死大鼠脑组织中的表达及其神经保护作用机制

张蓓|李亚军|张世俊|任会云|王敏娟|郭长江

西安医学院附属医院神经内科|陕西西安 710077

收稿日期:2012-07-26 发布日期:2012-11-28
通讯作者: 李亚军(Tel：029-84277893，E-mail：liyajun9@hotmail.com) E-mail:liyajun9@hotmail.com
作者简介:张蓓(1980-)|女|山东省济宁市人|主治医师|讲师|在读医学博士|主要从事脑血管病发病机制的研究。
基金资助:
陕西省自然科学基金资助课题(2010JM4054)；陕西省教育厅科研基金资助课题(09JK713)

Expression of neuroglobin in brain tissue of rats with cerebral infarction and its neuroprotective mechanisms

ZHANG Bei, LI Ya-jun, ZHANG Shi-jun, REN Hui-yun, WANG Min-juan, GUO Chang-jiang

Department of Neurology,Affiliated Hospital,Xi’[KG-*3]an Medical University,Xi’an 710077,China

Received:2012-07-26 Published:2012-11-28

摘要/Abstract

摘要：

目的：观察脑梗死大鼠脑组织中脑红蛋白（Ngb）的表达及其对PI3K/Akt 信号通路的影响，探讨二者在缺血性脑损伤中的作用及Ngb神经保护作用的可能机制。方法：60只SD大鼠随机分为假手术组、缺血组、Hemin（Ngb 诱导剂）组、LY（PI3-K/Akt抑制剂LY294002）组和Hemin＋LY组，每组12只。应用大脑中动脉线栓法制备脑梗死动物模型，术后24 h观察各组大鼠神经功能评分和脑组织梗死体积；应用RT-PCR法检测各组大鼠脑组织Ngb mRNA表达水平及PI3-K/Akt活性。结果：假手术组未见TTC不着色区，未检出神经功能缺损。与缺血组比较，Hemin组大鼠脑组织中Ngb和Akt mRNA表达水平升高（P<0.01），神经功能评分降低（P<0.05），脑梗死体积减小（P<0.01）；与缺血组比较，LY组大鼠脑梗死体积增加（P<0.01），神经功能评分升高（P<0.05），Akt mRNA表达水平降低（P<0.01），Ngb无明显变化。与Hemin组比较，Hemin＋LY组大鼠脑组织损伤加重，Akt mRNA表达水平明显降低（P<0.01），Ngb未被抑制。结论：Ngb可以减小局灶性脑缺血大鼠脑梗死体积，改善神经功能，并且可能通过PI3K/ Akt 信号通路发挥神经保护作用。

关键词: 脑红蛋白；磷脂酰肌醇-3激酶/ 丝氨酸-苏氨酸蛋白激酶；脑梗死

Abstract:

Objective To observe the expression of neuroglobin (Ngb)in brain tissue of rats with cerebral infarction and its effect on PI3-K/Akt signaling pathway,and to study their effects on cerebral ischemic injury and the possible mechanisms of the neuroprotective effect of Ngb.Methods 60 SD rats were randomly divided into sham operation group,ischemia group,and Hemin group,LY group,and Hemin＋LY group,and there were 12 rats in each group.Cerebral infarction models were established by middle cerebral artery thread embolism method.All rats were sacrificed 24 h after operation for neurological evaluation and detection of cerebralinfarction volume， and the expression levels of Ngb and Akt mRNA were detected by RT-PCR.Results There was not any area unstained by triphenyltetrazolium chloride and any neurologic impairment found in rats in sham operation group.Compared with ischemia group,the expression levels of Ngb and Akt mRNA in Hemin group were increased significantly（P<0.01）,with better neurological function(P<0.05) and less volume of cerebral infarction(P<0.01).The volume of cerebral infarction(P<0.01) and the neurological impairments （P<0.05）in LY group were increased,and the expression level of Akt mRNA in LY group was lower(P<0.01)than that in ischemia group,and there was no significant difference in the expression of Ngb (P>0.05).The cerebral tissue damage in Hemin＋LY group was aggrevated,and the expression level of Akt mRNA was decreased (P<0.01),while the level of Ngb mRNA was not inhibited by LY294002 (P>0.05) compared with Hemin group.Conclusion Ngb can reduce the voume of cerebral infarction and improve neurological function after focal cerebral ischemia in rats and its neuroprotective effect may be realized by activating PI3-K/Akt signaling pathway.

Key words: neuroglobin；phosphoinositide-3 kinase/serine-threonine kinase；cerebral infarction

中图分类号:

R743.33

张蓓|李亚军|张世俊|任会云|王敏娟|郭长江. 脑红蛋白在脑梗死大鼠脑组织中的表达及其神经保护作用机制[J]. J4, 2012, 38(6): 1114-1118.

ZHANG Bei, LI Ya-jun, ZHANG Shi-jun, REN Hui-yun, WANG Min-juan, GUO Chang-jiang. Expression of neuroglobin in brain tissue of rats with cerebral infarction and its neuroprotective mechanisms[J]. J4, 2012, 38(6): 1114-1118.