产志贺毒素大肠埃希菌中国分离株第1类整合子-基因盒的特征

doi:国家自然科学基金资助课题（30370074）

产志贺毒素大肠埃希菌中国分离株第1类整合子-基因盒的特征

李明成，王放，李凡

(吉林大学基础医学院病原生物学教研室吉林省病原生物学重点实验室，吉林长春 130021)

收稿日期:2006-09-04 修回日期:1900-01-01 出版日期:2007-05-28 发布日期:2007-05-28
通讯作者: 李明成

Characteristic of class 1 integron-cassette among Shiga toxin-producing E.coli isolates in China

LI Ming-cheng，WANG Fang，LI Fan

(Department of Pathogenobiology，School of Basic Medical Sciences，Jilin University，Key Laboratory of Pathogenobiology in Jilin Province，Changchun 130021,China)

Received:2006-09-04 Revised:1900-01-01 Online:2007-05-28 Published:2007-05-28
Contact: LI Fan

摘要/Abstract

摘要： 目的：探讨产志贺毒素大肠埃希菌中国分离株携带第1类整合子的特征及所含基因盒的种类。方法：采用纸片扩散法对8株产志贺毒素大肠埃希菌中国分离株进行抗生素耐药性监测；制备细菌转接合子，采用PCR技术鉴定第1类整合子，并对PCR产物测序及结果分析。结果：4株产志贺毒素大肠埃希菌对复合磺胺、四环素、氨苄西林、环丙沙星表现多重耐药，其中2株同时对链霉素和壮观霉素耐药。PCR检测第1类整合子1 000 bp 2株，PCR产物测序1 000 bp整合子携带腺苷酰基转移酶(aadA1)基因盒并存在于7 800 bp可接合质粒上，与sul 1和qacEΔ1基因连锁，分别传递着对链霉素、壮观霉素、复合磺胺和季胺盐类消毒剂的耐药性。结论: 产志贺毒素大肠埃希菌中国分离株可接合质粒上第1类整合子携带的aadA1耐药基因盒是该菌株对氨基糖苷类抗生素耐药产生和扩散的重要原因。

关键词: 大肠埃希菌, 整合子

Abstract: Abstract:Objective To explore the distribution and characteristic of class 1 integrons among Shiga toxin-producing E.coli (STEC) in China，and to elucidatethe type of cassettes.Methods Antibiotics susceptibility was tested by the disk diffusion method，class 1 integron was detected by PCR assay and PCR products were sequenced and analyzed in eight isolates. Results Four isolates were multiple-drug resistant whose antibiogram were ampicillin，tetracycline，erythromycin，sulfamethoxazole/trimethoprim and ciprofloxacin，but two isolates conferred resistance of streptomycin and spectinomycin simultaneously.Only the two isolates carried class 1 integrons ranging in 1 000 bp which located on the plasmid of 7 800 bp.The sequenced PCR product demonstrated that the 1 000 bp integron harbored aad A1 gene cassettes conferred the resistance of streptomycin and spectinomycin; and linked with sul 1 and qacEΔ1 gene conferred the resistance of sulfamethoxazole and quaternary disinfectants. Conclusion The class 1 integrons exists among STEC isolates in China and determines the resistant antibiotics.

Key words: E.coli, class 1 integron

中图分类号:

R378.21

李明成，王放，李凡. 产志贺毒素大肠埃希菌中国分离株第1类整合子-基因盒的特征[J]. J4, 2007, 33(3): 405-408.

LI Ming-cheng，WANG Fang，LI Fan. Characteristic of class 1 integron-cassette among Shiga toxin-producing E.coli isolates in China[J]. J4, 2007, 33(3): 405-408.

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