关键词:  , 银杏叶提取物；抑菌作用；牙龈卟啉单胞菌；金黄色葡萄球菌；大肠埃希菌

Abstract:

Abstract:Objective To explore the antibacterial activity of extract of Ginkgo biloba leaves on  Porphyromonas gingivalis in vitro , and to provid  pharmacological reference    for developing a new type of antibacterial drugs in the treatment of periodontal disease. Methods  This experiment was divided into negative control group, imipenem  control group and different concentrations and  forms of extract of Ginkgo biloba leaves groups. Solvent extraction method was used to extract the extract of Ginkgo biloba leaves,punching method and test tube method were performed to detect the antibacterial activity of extract of Ginkgo biloba leaves in anaerobic environment in vitro and compared with Staphylococcus  aureus and E.coli. By observing the antibacterial ring diameter and determination of the minimum bacteriostasis concentration (MIC),  the antibacterial activities of extract of Ginkgo biloba leaves in vitro were measured.Results  In the experiment of bacteriostatic ring,Porphyromonas gingivalis was treated with  extract of Ginkgo biloba leaves, Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule  concentrate and 1∶4 diluent,the bacteriostatic ring diameters were decreased with the decreasing  of the concentration.The maximum bacteriostatic diameter  of Ginkgo biloba extract  was 16.5 mm, and the maximum bacteriostasis diameters   of Ginkgo biloba leaf tablet and soft capsule  were 15.3  and 14.5 mm, respectively; the bacteriostatic diameter of the exact of Ginkgo biloba leaves  was bigger than  those  of Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule (P < 0.05), but there  was no statistical difference of bacteriostatic diameter between Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule  (P> 0.05); E. coli and Staphylococcus  aureus  groups get the same results.   When the  concentration of extract of Ginkgo biloba leaves  was more  than 1.95 mg/L, there was no growth of Porphyromonas gingivalis  but  E. coli and Staphylococcus aureusa still grew; only  the concentrations of exact of Girkgo biloba leaves were  more than 6.25  and 12.5 mg/L, E. coli and Staphylococcus aureus didn’t  grow; the bacteriostatic effect of extract of Ginkgo biloba on Porphromonas gingivalis was better  than  E.coli   and Staphylococcus aureus.Conclusion  Extract of Ginkgo biloba  leaves has antibacterial effect on Porphyromonas gingivalis.

Key words: extract of Ginkgo biloba leaves；antibacterial activity；Porphyromonas gingivalis；Staphylococcus aureus；Escherichia , coli